Brand  (β version)

PDB ID: 3K8L

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
EDO 1,2-ethanediol PoSSuM
BGC Beta-D-glucopyranose PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3K8L   PDBj   RCSB PDB   PDBe
Header : MEMBRANE PROTEIN
Title : Crystal structure of SusG-D498N mutant with maltoheptaose
Release Data : 2010-03-02
Compound :
mol_id molecule chains
1 Alpha-amylase, susG A,B
mutation: D498N
Source :
mol_id organism_scientific expression_system
1 Bacteroides thetaiotaomicron  (taxid:818) Escherichia coli  (taxid:562)
strain: VPI-5482
gene: BT_3698, SusG
expression_system_strain: Rosetta(DE3)pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET-28rTEV
Authors : Koropatkin, N.M., Smith, T.J.
Keywords : amylase, alpha8/beta8 barrel, CBM, beta-sandwich, MEMBRANE PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.300 Å )
Citation :

SusG: A Unique Cell-Membrane-Associated alpha-Amylase from a Prominent Human Gut Symbiont Targets Complex Starch Molecules.

Koropatkin, N.M.,Smith, T.J.
(2010)  Structure  18 : 200 - 215

PubMed: 20159465
DOI: 10.1016/j.str.2009.12.010

Reaction

Chain : A, B
UniProt : Q8A1G3 (SUSG_BACTN)
Reaction: EC: Evidence:
Physiological Direction:
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D- glucose units. 3.2.1.1 PubMed:20159465
-
Cofactor: Evidence: Note:
Ca(2+) ECO:0000269 | PubMed:20159465
Binds 1 Ca(2+) ion per subunit.