Brand  (β version)

PDB ID: 3K2P

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
JTH 2,7-dihydroxy-4-(propan-2-yl)cyclohepta-2,4,6-trien-1-one PoSSuM
Non-standard Residues
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Structure summary

Code : 3K2P   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : HIV-1 Reverse Transcriptase Isolated RnaseH Domain with the Inhibitor beta-thujaplicinol Bound at the Active Site
Release Data : 2010-02-09
Compound :
mol_id molecule chains synonym
1 Reverse Transcriptase A,B Reverse transcriptase/ribonuclease H, p66 RT
ec: 3.1.26.4
Source :
mol_id organism_scientific organism_common expression_system
1 Human immunodeficiency virus type 1  (taxid:11678) HIV-1 Escherichia coli  (taxid:562)
strain: BH10
gene: gag-pol
expression_system_vector_type: Plasmid
Authors : Pauly, T.A., Himmel, D.M., Maegley, K., Arnold, E.
Keywords : RNAse H inhibitor, reverse transcriptase, AIDS, HIV, protein-inhibitor complex, structure-based drug design, tropolones, tropylium ion, divalent cation chelator, metal-binding, RNA-binding, hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.04 Å )
Citation :

Structure of HIV-1 reverse transcriptase with the inhibitor beta-Thujaplicinol bound at the RNase H active site.

Himmel, D.M.,Maegley, K.A.,Pauly, T.A.  et al.
(2009)  Structure  17 : 1625 - 1635

PubMed: 20004166
DOI: 10.1016/j.str.2009.09.016

Reaction

Chain : A, B
UniProt : P03366 (POL_HV1B1)
Reaction: EC: Evidence:
Physiological Direction:
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro. 3.4.23.16 PROSITE-ProRule:PRU00275
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3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid. 3.1.13.2 ECO:0000250
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[Reverse transcriptase/ribonuclease H]
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus. 		3.1.26.13 PubMed:2476069
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[Reverse transcriptase/ribonuclease H]
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 		2.7.7.49 PROSITE- ProRule:PRU00405, PubMed:2476069
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[Reverse transcriptase/ribonuclease H]
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 		2.7.7.7 PROSITE- ProRule:PRU00405, PubMed:2476069
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