Brand  (β version)

PDB ID: 3IWM

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Ligands
Code Name Style Show Link
Non-standard Residues
Code Name Show
02J 5-methyl-1,2-oxazole-3-carboxylic acid
PJE (E,4s)-4-azanyl-5-[(3s)-2-oxidanylidenepyrrolidin-3-yl]pent-2-enoic acid
Glycosylation
Code Name Emphasize
Modification
Code Name Show
010 Phenylmethanol

Structure summary

Code : 3IWM   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : The octameric SARS-CoV main protease
Release Data : 2010-07-21
Compound :
mol_id molecule chains synonym
1 3C-like proteinase A,B,C,D 3CL-PRO, 3CLp
ec: 3.4.22.-
mol_id molecule chains
2 N-[(5-METHYLISOXAZOL-3-YL)CARBONYL]ALANYL-L-VALYL-N~1~-((1R,2Z)-4-(BENZYLOXY)-4-OXO-1-{[(3R)-2-OXOPYRROLIDIN-3-YL]METHYL}BUT-2-ENYL)-L-LEUCINAMIDE H,F,G,E
Source :
mol_id organism_scientific organism_common expression_system
1 SARS coronavirus  (taxid:227859) SARS-CoV Escherichia coli  (taxid:562)
expression_system_strain: BL21 Rosetta(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET-28a
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Zhong, N., Zhang, S., Xue, F., Lou, Z., Rao, Z., Xia, B.
Keywords : SARS-CoV, main protease, octamer, active conformation, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 3.200 Å )
Citation :

Three-dimensional domain swapping as a mechanism to lock the active conformation in a super-active octamer of SARS-CoV main protease

Zhang, S.,Zhong, N.,Xue, F.  et al.
(2010)  Protein Cell  1 : 371 - 383

PubMed: 21203949
DOI: 10.1007/s13238-010-0044-8

Reaction

Chain : A, B, C, D
UniProt : P0C6U8 (R1A_SARS)
Reaction: EC: Evidence:
Physiological Direction:
[3C-like proteinase nsp5]
TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position. 		3.4.22.69 PubMed:12917450, PubMed:14561748
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[Papain-like protease nsp3]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal). 		3.4.19.12 PubMed:12917450, PubMed:17692280
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[RNA-capping enzyme subunit nsp9]
a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'- end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + diphosphate 		2.7.7.50 UniProtKB:P0DTC1
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