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Ligands
Code Name Style Show Link
CA Calcium ion
F42 Coenzyme f420
H4M 5,10-dimethylene tetrahydromethanopterin
NA Sodium ion
Non-standard Residues
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Glycosylation
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Code : 3IQE   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of F420 dependent methylene-tetrahydromethanopterin dehydrogenase in complex with methylene-tetrahydromethanopterin and coenzyme F420
Release Data : 2009-10-06
Compound :
mol_id molecule chains synonym
1 F420-dependent methylenetetrahydromethanopterin dehydrogenase A,B,C,D,E,F MTD, Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase
ec: 1.5.99.9
Source :
mol_id organism_scientific expression_system
1 Methanopyrus kandleri  (taxid:2320) Escherichia coli  (taxid:562)
gene: mtd
expression_system_strain: BL21 (DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET17b
Authors : Ceh, K.E., Demmer, U., Warkentin, E., Moll, J., Thauer, R.K., Shima, S., Ermler, U.
Keywords : ternary complex of protein, substrate and co-substrate, Methanogenesis, One-carbon metabolism, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.80 Å )
Citation :

Structural basis of the hydride transfer mechanism in F(420)-dependent methylenetetrahydromethanopterin dehydrogenase

Ceh, K.,Demmer, U.,Warkentin, E.  et al.
(2009)  Biochemistry  48 : 10098 - 10105

PubMed: 19761261
DOI: 10.1021/bi901104d

Chain : A, B, C, D, E, F
UniProt : P94951 (MTD_METKA)
Reaction: EC: Evidence:
Physiological Direction:
5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8- tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n) 1.5.98.1 -
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