Brand  (β version)

PDB ID: 3INV

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Ligands
Code Name Style Show Link
C50 1-[3-(2,3-dichlorophenoxy)propoxy]-6,6-dimethyl-1,6-dihydro-1,3,5-triazine-2,4-diamine PoSSuM
GOL Glycerol PoSSuM
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate PoSSuM
UMP 2'-deoxyuridine 5'-monophosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 3INV   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE/TRANSFERASE
Title : Trypanosoma cruzi Dihydrofolate Reductase-Thymidylate Synthase COMPLEXED WITH NADPH, dUMP AND C-448 ANTIFOLATE
Release Data : 2010-07-28
Compound :
mol_id molecule chains synonym
1 Bifunctional dihydrofolate reductase-thymidylate synthase A,B DHFR-TS, Dihydrofolate reductase, Thymidylate synthase
ec: 1.5.1.3, 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Trypanosoma cruzi  (taxid:5693) Escherichia coli  (taxid:469008)
strain: Y
expression_system_strain: BL21(DE3) pLysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET11c
Authors : Chitnumsub, P., Yuvaniyama, J., Yuthavong, Y.
Keywords : DHFR-TS antifolate complex, Methyltransferase, Multifunctional enzyme, NADP, Nucleotide biosynthesis, One-carbon metabolism, Oxidoreductase, Transferase, OXIDOREDUCTASE-TRANSFERASE COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.37 Å )
Citation :

Structural basis of antifolate inhibition of Trypanosoma cruzi Dihydrofolate Reductase-Thymidylate Synthase

Chitnumsub, P.,Yuvaniyama, J.,Vilaivan, T.  et al.
To be Published 

Reaction

Chain : A, B
UniProt : Q27793 (DRTS_TRYCR)
Reaction: EC: Evidence:
Physiological Direction:
(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 -
-
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 -
-