Brand  (β version)

PDB ID: 3IA2

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
J6Z (2r)-butane-2-sulfonate PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3IA2   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Pseudomonas fluorescens esterase complexed to the R-enantiomer of a sulfonate transition state analog
Release Data : 2010-07-21
Compound :
mol_id molecule chains synonym
1 Arylesterase A,B,C,D,E,F Aryl-ester hydrolase, PFE, Putative bromoperoxidase
ec: 3.1.1.2, 1.-.-.-
Source :
mol_id organism_scientific expression_system
1 Pseudomonas fluorescens  (taxid:294) Escherichia coli  (taxid:562)
expression_system_strain: DH5alpha
expression_system_vector_type: plasmid
expression_system_plasmid: pJOE2792
Authors : Schrag, J.D., Kazlauskas, R.J., Jiang, Y., Morley, K.
Keywords : alpha-beta hydrolase fold, transition state analog, Hydrolase, Oxidoreductase, Peroxidase
Exp. method : X-RAY DIFFRACTION ( 1.65 Å )
Citation :

Different active-site loop orientation in serine hydrolases versus acyltransferases.

Jiang, Y.,Morley, K.L.,Schrag, J.D.  et al.
(2011)  Chembiochem  12 : 768 - 776

PubMed: 21351219
DOI: 10.1002/cbic.201000693

Reaction

Chain : A, B, C, D, E, F
UniProt : P22862 (ESTE_PSEFL)
Reaction: EC: Evidence:
Physiological Direction:
a phenyl acetate + H2O = a phenol + acetate + H(+) 3.1.1.2 PubMed:1368608, PubMed:15803517, PubMed:7704276, PubMed:9571805
-
H2O + peracetic acid = acetate + H(+) + H2O2 - PubMed:15803517, PubMed:20112920
-
a percarboxylic acid + H2O = a carboxylate + H(+) + H2O2 - PubMed:15803517, PubMed:20112920, PubMed:22618813
-