Brand  (β version)

PDB ID: 3I3D

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Ligands
Code Name Style Show Link
DMS Dimethyl sulfoxide PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
IPT 1-methylethyl 1-thio-beta-D-galactopyranoside PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3I3D   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : E. COLI (lacZ) BETA-GALACTOSIDASE (M542A) IN COMPLEX WITH IPTG
Release Data : 2010-05-12
Compound :
mol_id molecule chains
1 Beta-galactosidase A,B,C,D
ec: 3.2.1.23
fragment: Residues 10-1024
mutation: M543A
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
gene: lacz
expression_system_strain: LMG104
expression_system_vector_type: PLASMID
expression_system_plasmid: pBAD/HIS/LacZ
Authors : Dugdale, M.L., Dymianiw, D., Minhas, B., Huber, R.E.
Keywords : BETA-GALACTOSIDASE, TIM BARREL (ALPHA/BETA BARREL), JELLY-ROLL BARREL, IMMUNOGLOBULIN BETA SUPERSANDWHICH, Glycosidase, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Role of Met-542 as a guide for the conformational changes of Phe-601 that occur during the reaction of β-galactosidase (Escherichia coli).

Dugdale, M.L.,Dymianiw, D.L.,Minhas, B.K.  et al.
(2010)  Biochem.Cell Biol.  88 : 861 - 869

PubMed: 20921997
DOI: 10.1139/O10-009

Reaction

Chain : A, B, C, D
UniProt : B8LFD6 (B8LFD6_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. 3.2.1.23 ARBA:ARBA00001412, HAMAP- Rule:MF_01687
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