Brand  (β version)

PDB ID: 3HXY

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Ligands
Code Name Style Show Link
5AL 5'-O-[(R)-{[(2s)-2-aminopropanoyl]oxy}(hydroxy)phosphoryl]adenosine PoSSuM
ACP Phosphomethylphosphonic acid adenylate ester PoSSuM
BME Beta-mercaptoethanol PoSSuM
HED 2-hydroxyethyl disulfide PoSSuM
MDN Methylenediphosphonic acid PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3HXY   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Crystal Structure of catalytic fragment of E. coli AlaRS in complex with AMPPCP, Ala-AMP and PCP
Release Data : 2009-12-15
Compound :
mol_id molecule chains synonym
1 Alanyl-tRNA synthetase A Alanine-tRNA ligase, AlaRS
ec: 6.1.1.7
fragment: N-terminal Catalytic fragment residues 2-442
mutation: H104L, Q108L, E112L
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K-12
gene: alaS, lovB, b2697, JW2667
expression_system_strain: BL21(DE3) CondonPlus
expression_system_vector_type: plasmid
expression_system_plasmid: pET20b+
Authors : Guo, M., Yang, X.-L., Schimmel, P.
Keywords : Aminoacyl-tRNA synthetase, Ligase, Protein biosynthesis, Nucleotide-binding, amino acid-binding, ATP-binding, Metal-binding, Zinc-finger
Exp. method : X-RAY DIFFRACTION ( 2.27 Å )
Citation :

Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.

Guo, M.,Chong, Y.E.,Shapiro, R.  et al.
(2009)  Nature  462 : 808 - 812

PubMed: 20010690
DOI: 10.1038/nature08612

Reaction

Chain : A
UniProt : P00957 (SYA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl- tRNA(Ala) 6.1.1.7 PubMed:7005211
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