Brand  (β version)

PDB ID: 3HLW

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CE3 (6r,7r)-3-(acetyloxymethyl)-7-[[(2z)-2-(2-amino-1,3-thiazol-4-yl)-2-methoxyimino-ethanoyl]amino]-8-oxo-5-thia-1-azabicy clo[4.2.0]oct-2-ene-2-carboxylic acid PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3HLW   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : CTX-M-9 S70G in complex with cefotaxime
Release Data : 2010-06-02
Compound :
mol_id molecule chains synonym
1 CTX-M-9 extended-spectrum beta-lactamase A,B Beta-lactamase, Beta-lactamase CTX-M-9a, Betalactamase CTX-M-9, CTX-M-9 beta-lactamase
fragment: UNP residues 29-291
mutation: S70G
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: blaCTX-M-9, blaCTX-M-9a, blaCTX-M-9b
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET9 derivative
Authors : Delmas, J., Leyssne, D., Dubois, D., Vazeille, E., Robin, F., Bonnet, R.
Keywords : beta-lactamase, ESBL, CTX-M, CTX-M-9, beta-lactam, cephalosporin, cefotaxime, michaelis, complex, Antibiotic resistance, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

Structural insights into substrate recognition and product expulsion in CTX-M enzymes.

Delmas, J.,Leyssene, D.,Dubois, D.  et al.
(2010)  J.Mol.Biol.  400 : 108 - 120

PubMed: 20452359
DOI: 10.1016/j.jmb.2010.04.062

Reaction

Chain : A, B
UniProt : Q9L5C8 (Q9L5C8_ECOLX)
Reaction: EC: Evidence:
Physiological Direction:
a beta-lactam + H2O = a substituted beta-amino acid 3.5.2.6 ARBA:ARBA00001526, RuleBase:RU361140
-