Brand  (β version)

PDB ID: 3HAY

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Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FHU (5s,6r)-5-fluoro-6-hydroxy-pseudouridine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 3HAY   PDBj   RCSB PDB   PDBe
Header : ISOMERASE/BIOSYNTHETIC PROTEIN/RNA
Title : Crystal structure of a substrate-bound full H/ACA RNP from Pyrococcus furiosus
Release Data : 2009-06-23
Compound :
mol_id molecule chains synonym
1 Probable tRNA pseudouridine synthase B A tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase
ec: 5.4.99.-
mol_id molecule chains synonym
2 Small nucleolar rnp gar1-like protein B Gar1
mol_id molecule chains
3 Ribosome biogenesis protein Nop10 C
mol_id molecule chains
4 50S ribosomal protein L7Ae D
mol_id molecule chains
5 H/ACA RNA E
mol_id molecule chains
6 5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3' F
Source :
mol_id organism_scientific expression_system
1 Pyrococcus furiosus  (taxid:2261) Escherichia coli  (taxid:562)
gene: truB, PF1785
mol_id organism_scientific expression_system
2 Pyrococcus furiosus  (taxid:2261) Escherichia coli  (taxid:562)
gene: PF1791
mol_id organism_scientific expression_system
3 Pyrococcus furiosus  (taxid:2261) Escherichia coli  (taxid:562)
gene: PF1141
mol_id organism_scientific expression_system
4 Pyrococcus furiosus  (taxid:2261) Escherichia coli  (taxid:562)
gene: rpl7ae, PF1367
mol_id organism_scientific
5
synthetic: yes
other_details: RNA WAS PREPARED BY IN VITRO TRANSCRIPTION
mol_id organism_scientific
6
synthetic: yes
Authors : Ye, K.
Keywords : H/ACA, guide RNA, RNA-protein complex, pseudouridine synthase, Isomerase, tRNA processing, Ribonucleoprotein, Ribosome biogenesis, rRNA processing, Ribosomal protein, RNA-binding, ISOMERASE-BIOSYNTHETIC PROTEIN-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 4.99 Å )
Citation :

Structural mechanism of substrate RNA recruitment in H/ACA RNA-guided pseudouridine synthase.

Duan, J.,Li, L.,Lu, J.  et al.
(2009)  Mol.Cell  34 : 427 - 439

PubMed: 19481523
DOI: 10.1016/j.molcel.2009.05.005

Reaction

Chain : A
UniProt : Q7LWY0 (TRUB_PYRFU)
Reaction: EC: Evidence:
Physiological Direction:
uridine(55) in tRNA = pseudouridine(55) in tRNA 5.4.99.25 HAMAP-Rule:MF_01081
-
Chain : B
UniProt : Q8U029 (Q8U029_PYRFU)
Reaction : -
Chain : C
UniProt : Q8U1R4 (NOP10_PYRFU)
Reaction : -
Chain : D
UniProt : Q8U160 (RL7A_PYRFU)
Reaction : -