Brand  (β version)

PDB ID: 3H65

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Ligands
Code Name Style Show Link
CMO Carbon monoxide PoSSuM
DTV (2s,3s)-1,4-dimercaptobutane-2,3-diol PoSSuM
FE2 Fe (II) ion PoSSuM
H4M 5,10-dimethylene tetrahydromethanopterin PoSSuM
I2C 5'-O-[(S)-hydroxy{[2-hydroxy-3,5-dimethyl-6-(2-oxoethyl)pyridin-4-yl]oxy}phosphoryl]guanosine PoSSuM
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Structure summary

Code : 3H65   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : The Crystal Structure of C176A Mutated [Fe]-Hydrogenase (Hmd) Holoenzyme in Complex with Methylenetetrahydromethanopterin
Release Data : 2009-09-01
Compound :
mol_id molecule chains synonym
1 5,10-methenyltetrahydromethanopterin hydrogenase A H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase, N(5),N(10)-methenyltetrahydromethanopterin hydrogenase, H(2)-dependent methylene-H(4)MPT dehydrogenase
ec: 1.12.98.2
mutation: C176A
Source :
mol_id organism_scientific organism_common expression_system
1 Methanocaldococcus jannaschii  (taxid:2190) Methanococcus jannaschii Escherichia coli  (taxid:562)
strain: DSM 2661
gene: hmd, MJ0784
expression_system_strain: HMS174(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET-24b(+)
Authors : Hiromoto, T., Warkentin, E., Shima, S., Ermler, U.
Keywords : ROSSMANN FOLD, HELIX BUNDLE, COMPLEX WITH IRON GUANYLYL PYRIDINOL COFACTOR, C176A MUTANT, COMPLEX WITH METHYLENETETRAHYDROMETHANOPTERIN, Methanogenesis, One-carbon metabolism, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 2.150 Å )
Citation :

The crystal structure of an [Fe]-hydrogenase-substrate complex reveals the framework for H2 activation.

Hiromoto, T.,Warkentin, E.,Moll, J.  et al.
(2009)  Angew.Chem.Int.Ed.Engl.  48 : 6457 - 6460

PubMed: 19623593
DOI: 10.1002/anie.200902695

Reaction

Chain : A
UniProt : Q58194 (HMD_METJA)
Reaction: EC: Evidence:
Physiological Direction:
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10- methylenetetrahydromethanopterin + H(+) 1.12.98.2 -
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