Brand  (β version)

PDB ID: 3GLT

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CO3 Carbonate ion PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
FZN (2s)-2-amino-6-{[(1z)-1-{[(2r,3r,4s,5r)-5-({[(R)-{[(R)-{[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}methyl)-3,4-dihydroxytetrahydrofuran-2-yl]sulfanyl}ethylidene]amino}hexanoic acid
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3GLT   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE REGULATOR
Title : Crystal Structure of Human SIRT3 with ADPR bound to the AceCS2 peptide containing a thioacetyl lysine
Release Data : 2009-06-16
Compound :
mol_id molecule chains synonym
1 NAD-dependent deacetylase sirtuin-3, mitochondrial A SIR2-like protein 3, hSIRT3
ec: 3.5.1.-
fragment: Human SIRT3, residues 118-399
mol_id molecule chains synonym
2 Acetyl-coenzyme A synthetase 2-like, mitochondrial B Acetate--CoA ligase 2, Acetyl-CoA synthetase 2, Acyl-CoA synthetase short-chain family member 1
ec: 6.2.1.1
fragment: AceCS2 peptide, residues 638-649
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: SIR2L3, SIRT3
expression_system_strain: BL21-GOLD(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: modified pET21b
mol_id organism_scientific organism_common
2 Homo sapiens  (taxid:9606) Human
synthetic: yes
Authors : Jin, L., Wei, W., Jiang, Y., Peng, H., Cai, J., Mao, C., Dai, H., Bemis, J.E., Jirousek, M.R., Milne, J.C., Westphal, C.H., Perni, R.B.
Keywords : NAD dependent deacetylase, sirtuin, intermediate trapped structure, thioacetyl peptide, Hydrolase, Metal-binding, Mitochondrion, NAD, Polymorphism, Transit peptide, Zinc, Alternative splicing, Ligase, HYDROLASE-HYDROLASE REGULATOR COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Crystal Structures of Human SIRT3 Displaying Substrate-induced Conformational Changes.

Jin, L.,Wei, W.,Jiang, Y.  et al.
(2009)  J.Biol.Chem.  284 : 24394 - 24405

PubMed: 19535340
DOI: 10.1074/jbc.M109.014928

Reaction

Chain : A
UniProt : Q9NTG7 (SIR3_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl- ADP-D-ribose + L-lysyl-[protein] + nicotinamide 2.3.1.286 PROSITE-ProRule:PRU00236, PubMed:12186850, PubMed:12374852, PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:19535340, PubMed:24121500, PubMed:23283301
-
Chain : B
UniProt : Q9NUB1 (ACS2L_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate 6.2.1.1 PubMed:16788062
left-to-right
ATP + CoA + propanoate = AMP + diphosphate + propanoyl-CoA 6.2.1.17 UniProtKB:Q99NB1
left-to-right