Brand  (β version)

PDB ID: 3G5N

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Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
PB2 1-(biphenyl-4-ylmethyl)-1h-imidazole PoSSuM
CM5 5-cyclohexyl-1-pentyl-beta-D-maltoside PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3G5N   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Triple ligand occupancy crystal structure of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole
Release Data : 2009-05-12
Compound :
mol_id molecule chains synonym
1 Cytochrome P450 2B4 A,B,C,D CYPIIB4, P450-LM2, Isozyme 2, P450 types B0 and B1
ec: 1.14.14.1
mutation: E2A, G22K, H23K, P24T, K25S, A26S, H27K, R29K, P221S, H226Y
Source :
mol_id organism_scientific organism_common expression_system
1 Oryctolagus cuniculus  (taxid:9986) European rabbit,Japanese white rabbit,domestic rabbit,rabbits Escherichia coli  (taxid:562)
gene: CYP2B4
expression_system_strain: TOPP3
expression_system_vector_type: plasmid
expression_system_plasmid: PKK
Authors : Gay, S.C., Sun, L., Maekawa, K., Halpert, J.R., Stout, C.D.
Keywords : P450, cytochrome P450 2B4, monooxygenase, oxidoreductase, membrane protein, CYP 2B4, CYP LM2, Endoplasmic reticulum, Heme, Iron, Membrane, Metal-binding, Microsome, Phosphoprotein, Polymorphism
Exp. method : X-RAY DIFFRACTION ( 2.500 Å )
Citation :

Crystal structures of cytochrome P450 2B4 in complex with the inhibitor 1-biphenyl-4-methyl-1H-imidazole: ligand-induced structural response through alpha-helical repositioning.

Gay, S.C.,Sun, L.,Maekawa, K.  et al.
(2009)  Biochemistry  48 : 4762 - 4771

PubMed: 19397311
DOI: 10.1021/bi9003765

Structural and thermodynamic consequences of 1-(4-chlorophenyl)imidazole binding to cytochrome P450 2B4.

Zhao, Y.,Sun, L.,Muralidhara, B.K.  et al.
(2007)  Biochemistry  46 : 11559 - 11567

Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9 {angstrom} resolution: Insight into the range of P450 conformations and coordination of redox partner binding.

Scott, E.E.,White, M.A.,He, Y.A.  et al.
(2004)  J.Biol.Chem.  279 : 27294 - 27301

Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: insight into P450 conformational plasticity and membrane interaction.

Zhao, Y.,White, M.A.,Muralidhara, B.K.  et al.
(2006)  J.Biol.Chem.  281 : 5973 - 5981

An open conformation of mammalian cytochrome P450 2B4 at 1.6 A resolution.

Scott, E.E.,He, Y.A.,Wester, M.R.  et al.
(2003)  Proc.Natl.Acad.Sci.USA  100 : 13196 - 13201

Reaction

Chain : A, B, C, D
UniProt : P00178 (CP2B4_RABIT)
Reaction: EC: Evidence:
Physiological Direction:
an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] 1.14.14.1 -
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