Brand  (β version)

PDB ID: 3FUC

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Ligands
Code Name Style Show Link
9D9 [5-(2-amino-4-oxo-4,5-dihydro-3h-pyrrolo[3,2-D]pyrimidin-7-yl)-1,1-difluoropentyl]phosphonic acid PoSSuM
AZI Azide ion PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3FUC   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Recombinant calf purine nucleoside phosphorylase in a binary complex with multisubstrate analogue inhibitor 9-(5',5'-difluoro-5'-phosphonopentyl)-9-deazaguanine structure in a new space group with one full trimer in the asymmetric unit
Release Data : 2009-12-08
Compound :
mol_id molecule chains synonym
1 Purine nucleoside phosphorylase A,B,C PNP, Inosine phosphorylase
ec: 2.4.2.1
fragment: residues 1-284
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Bovine Escherichia coli  (taxid:562)
gene: NP, PNP
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET-28A+
Authors : Bochtler, M., Breer, K., Bzowska, A., Chojnowski, G., Hashimoto, M., Hikishima, S., Narczyk, M., Wielgus-Kutrowska, B., Yokomatsu, T.
Keywords : purine nucleoside phosphorylase, recombinant, glycosyltransferase, transferase, 9-deazaguanine, multisubstrate analogue inhibitors, nucleoside-binding, phosphate-binding, binding site, trimer
Exp. method : X-RAY DIFFRACTION ( 1.45 Å )
Citation :

1.45 A resolution crystal structure of recombinant PNP in complex with a pM multisubstrate analogue inhibitor bearing one feature of the postulated transition state.

Chojnowski, G.,Breer, K.,Narczyk, M.  et al.
(2010)  Biochem.Biophys.Res.Commun.  391 : 703 - 708

PubMed: 19944078
DOI: 10.1016/j.bbrc.2009.11.124

Reaction

Chain : A, B, C
UniProt : P55859 (PNPH_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine 2.4.2.1 UniProtKB:P00491
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guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine 2.4.2.1 UniProtKB:P00491
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2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1- phosphate + guanine 2.4.2.1 UniProtKB:P23492
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2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1- phosphate + hypoxanthine 2.4.2.1 UniProtKB:P23492
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