Brand  (β version)

PDB ID: 3FL1

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
CGP 2'-deoxycytidine-2'-deoxyguanosine-3',5'-monophosphate PoSSuM
SO4 Sulfate ion PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
Code Name Show
YCM S-(2-amino-2-oxoethyl)-L-cysteine
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3FL1   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : X-ray structure of the non covalent swapped form of the A19P/Q28L/K31C/S32C mutant of bovine pancreatic ribonuclease in complex with 2'-DEOXYCYTIDINE-2'-DEOXYGUANOSINE-3',5'-MONOPHOSPHATE
Release Data : 2009-03-24
Compound :
mol_id molecule chains synonym
1 Ribonuclease pancreatic A,B ribonuclease, RNase 1, RNase A
ec: 3.1.27.5
mutation: A19P, Q28L, K31C, S32C
Source :
mol_id organism_scientific organism_common expression_system
1 Bos taurus  (taxid:9913) Bovine Escherichia coli  (taxid:562)
gene: RNASE1, RNS1
expression_system_vector_type: plasmid
expression_system_plasmid: pET22b(+)
Authors : Merlino, A., Russo Krauss, I., Perillo, M., Mattia, C.A., Ercole, C., Picone, D., Vergara, A., Sica, F.
Keywords : 3D-domain swapping, bovine seminal ribonuclease, non-covalent dimer, antitumor activity, quaternary structure flexibility, protein mutations and evolution, Endonuclease, Glycation, Glycoprotein, Hydrolase, Nuclease, Secreted
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Toward an antitumor form of bovine pancreatic ribonuclease: The crystal structure of three noncovalent dimeric mutants

Merlino, A.,Russo Krauss, I.,Perillo, M.  et al.
(2009)  Biopolymers  91 : 1029 - 1037

PubMed: 19280639
DOI: 10.1002/bip.21183

Structure and stability of the non-covalent swapped dimer of bovine seminal ribonuclease: an enzyme tailored to evade ribonuclease protein inhibitor

Sica, F.,Di Fiore, A.,Merlino, A.  et al.
(2004)  J.Biol.Chem.  279 : 36753 - 36760

PubMed: 15192098
DOI: 10.1074/jbc.M405655200

The buried diversity of bovine seminal ribonuclease: shape and cytotoxicity of the swapped non-covalent form of the enzyme

Merlino, A.,Ercole, C.,Picone, D.  et al.
(2008)  J.Mol.Biol.  376 : 427 - 437

PubMed: 18164315
DOI: 10.1016/j.jmb.2007.11.008

Hints on the evolutionary design of a dimeric RNase with special bioactions

Di Donato, A.,Cafaro, V.,Romeo, I.  et al.
(1995)  Protein Sci.  4 : 1470 - 1477

PubMed: 8520472

Reaction

Chain : A, B
UniProt : P61823 (RNAS1_BOVIN)
Reaction: EC: Evidence:
Physiological Direction:
an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'- phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. 4.6.1.18 -
-
an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'- phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]. 4.6.1.18 -
-