Brand  (β version)

PDB ID: 3E9Y

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Ligands
Code Name Style Show Link
1MS N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide PoSSuM
NHE 2-[N-cyclohexylamino]ethane sulfonic acid PoSSuM
MG Magnesium ion PoSSuM
TDM 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyl trihydrogen diphosphate PoSSuM
FAB Flavin-adenine dinucleotide-n5-isobutyl ketone PoSSuM
Non-standard Residues
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CSD 3-sulfinoalanine
Glycosylation
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Structure summary

Code : 3E9Y   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron
Release Data : 2009-03-31
Compound :
mol_id molecule chains synonym
1 Acetolactate synthase, chloroplastic A Acetohydroxy-acid synthase, ALS
ec: 2.2.1.6
fragment: residues 87-670
Source :
mol_id organism_scientific organism_common expression_system
1 Arabidopsis thaliana  (taxid:3702) Mouse-ear cress Escherichia coli  (taxid:562)
gene: CSR 1.2, At3g48560, T8P19.70
expression_system_strain: BL21
expression_system_vector_type: Plasmid
expression_system_plasmid: pET30
Authors : Guddat, L.W., Duggleby, R.G., Wang, J.-G., Li, Z.-M.
Keywords : Protein-FAD-HEThDP complex, Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Chloroplast, FAD, Flavoprotein, Herbicide resistance, Magnesium, Metal-binding, Thiamine pyrophosphate, Transferase, Transit peptide
Exp. method : X-RAY DIFFRACTION ( 3.00 Å )
Citation :

Crystal structures of two novel sulfonylurea herbicides in complex with Arabidopsis thaliana acetohydroxyacid synthase.

Wang, J.-G.,Lee, P.K.,Dong, Y.-H.  et al.
(2009)  Febs J.  276 : 1282 - 1290

PubMed: 19187232
DOI: 10.1111/j.1742-4658.2009.06863.x

Reaction

Chain : A
UniProt : P17597 (ILVB_ARATH)
Reaction: EC: Evidence:
Physiological Direction:
2 pyruvate + H(+) = (2S)-2-acetolactate + CO2 2.2.1.6 -
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:32640464
Binds 1 Mg(2+) ion per subunit.
FAD ECO:0000269 | PubMed:32640464
Binds 1 Mg(2+) ion per subunit.
thiamine diphosphate ECO:0000269 | PubMed:32640464
Binds 1 thiamine pyrophosphate per subunit.