Brand  (β version)

PDB ID: 3DYO

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
DMS Dimethyl sulfoxide PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
IPT 1-methylethyl 1-thio-beta-D-galactopyranoside PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3DYO   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : E. coli (lacZ) beta-galactosidase (H418N) in complex with IPTG
Release Data : 2008-10-28
Compound :
mol_id molecule chains
1 Beta-galactosidase A,B,C,D
ec: 3.2.1.23
mutation: H418N
Source :
mol_id organism_scientific expression_system
1 Escherichia coli K12  (taxid:83333) Escherichia coli  (taxid:562)
gene: lacZ, b0344, JW0335
expression_system_strain: JM109
expression_system_vector_type: PLASMID
expression_system_plasmid: pET15b
Authors : Juers, D.H., Huber, R.E., Matthews, B.W.
Keywords : beta-galactosidase, Glycosidase, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.8 Å )
Citation :

Direct and indirect roles of His-418 in metal binding and in the activity of beta-galactosidase (E. coli).

Juers, D.H.,Rob, B.,Dugdale, M.L.  et al.
(2009)  Protein Sci.  18 : 1281 - 1292

PubMed: 19472413
DOI: 10.1002/pro.140

Reaction

Chain : A, B, C, D
UniProt : P00722 (BGAL_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. 3.2.1.23 -
-
Cofactor: Evidence: Note:
Mn(2+) - Binds 2 magnesium ions per monomer. Can also use manganese.
Na(+) - Binds 1 sodium ion per monomer.