Brand  (β version)

PDB ID: 3D9G

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Ligands
Code Name Style Show Link
CNX Heptanenitrile PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
GOL Glycerol PoSSuM
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Structure summary

Code : 3D9G   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Nitroalkane oxidase: wild type crystallized in a trapped state forming a cyanoadduct with FAD
Release Data : 2009-04-07
Compound :
mol_id molecule chains
1 Nitroalkane oxidase A,B,C,D
ec: 1.7.3.1
Source :
mol_id organism_scientific expression_system
1 Fusarium oxysporum  (taxid:5507) Escherichia coli  (taxid:562)
expression_system_strain: Bl21(de3)
expression_system_vector_type: plasmid
expression_system_plasmid: PETNAO4
Authors : Heroux, A., Bozinovski, D.M., Valley, M.P., Fitzpatrick, P.F., Orville, A.M.
Keywords : OXIDOREDUCTASE, FLAVOENZYME, NITROALKANE, ACYL-COA DEHYDROGENASE, LONG CELL EDGE, FAD, INHIBITOR, FLAVOPROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.150 Å )
Citation :

Crystal structures of intermediates in the nitroalkane oxidase reaction.

Heroux, A.,Bozinovski, D.M.,Valley, M.P.  et al.
(2009)  Biochemistry  48 : 3407 - 3416

PubMed: 19265437
DOI: 10.1021/bi8023042

Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover.

Nagpal, A.,Valley, M.P.,Fitzpatrick, P.F.  et al.
(2006)  Biochemistry  45 : 1138 - 1150

PubMed: 16430210
DOI: 10.1021/bi051966w

Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase.

Fitzpatrick, P.F.,Bozinovski, D.M.,Heroux, A.  et al.
(2007)  Biochemistry  46 : 13800 - 13808

PubMed: 17994768
DOI: 10.1021/bi701557k

Reaction

Chain : A, B, C, D
UniProt : Q8X1D8 (NAO_FUSOX)
Reaction: EC: Evidence:
Physiological Direction:
a primary nitroalkane + H2O + O2 = an aldehyde + H(+) + H2O2 + nitrite 1.7.3.1 PubMed:11867731, PubMed:12862464, PubMed:17994768, PubMed:19265437, PubMed:22538
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a secondary nitroalkane + H2O + O2 = a ketone + H(+) + H2O2 + nitrite 1.7.3.1 PubMed:11867731, PubMed:12862464, PubMed:17994768, PubMed:19265437, PubMed:22538
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