Brand  (β version)

PDB ID: 3D54

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
ADP Adenosine-5'-diphosphate PoSSuM
NA Sodium ion PoSSuM
Modified Residues
Code Name Link
CYG 2-amino-4-(amino-3-oxo-propylsulfanylcarbonyl)-butyric acid PoSSuM

Structure summary

Code : 3D54
Header : LIGASE
Title : Structure of PurLQS from Thermotoga maritima
Release Data : 2008-07-22
Compound :
mol_id molecule chains synonym
1 Phosphoribosylformylglycinamidine synthase II A,E,I FGAM synthase II
ec: 6.3.5.3
mol_id molecule chains
2 Formylglycinamide ribonucleotide amidotransferase B,C,F,G,J,K
ec: 6.3.5.3
mol_id molecule chains synonym
3 Phosphoribosylformylglycinamidine synthase 1 D,H,L Phosphoribosylformylglycinamidine synthase I, FGAM synthase I
ec: 6.3.5.3
Source :
mol_id organism_scientific expression_system
1 Thermotoga maritima  (taxid:2336) Escherichia coli
gene: purL
expression_system_vector_type: plasmid
mol_id organism_scientific expression_system
2 Thermotoga maritima  (taxid:2336) Escherichia coli
mol_id organism_scientific expression_system
3 Thermotoga maritima  (taxid:2336) Escherichia coli
gene: purQ
Authors : Ealick, S.E., Morar, M.
Keywords : alpha-beta structure, ATP-binding, Ligase, Nucleotide-binding, Purine biosynthesis, Glutamine amidotransferase, LIGASE-UNKNOWN FUNCTION COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.50 Å )
Citation :

Formylglycinamide ribonucleotide amidotransferase from Thermotoga maritima: structural insights into complex formation.

Morar, M.,Hoskins, A.A.,Stubbe, J.  et al.
(2008)  Biochemistry  47 : 7816 - 7830

PubMed: 18597481
DOI: 10.1021/bi800329p

Reaction

Chain : A, E, I
UniProt : Q9X0X3 (PURL_THEMA)
Reaction : ATP + N(2)-formyl-N(1)-(5-phospho-D- ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2- (formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Chain : B, C, F, G, J, K
UniProt : Q9X0X1 (PURS_THEMA)
Reaction : ATP + N(2)-formyl-N(1)-(5-phospho-D- ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2- (formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Chain : D, H, L
UniProt : Q9X0X2 (PURQ_THEMA)
Reaction : ATP + N(2)-formyl-N(1)-(5-phospho-D- ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2- (formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
L-glutamine + H(2)O = L-glutamate + NH(3).