Brand  (β version)

PDB ID: 3CUS

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Ligands
Code Name Style Show Link
SF4 Iron/sulfur cluster PoSSuM
F3S Fe3-s4 cluster PoSSuM
GOL Glycerol PoSSuM
NI Nickel (II) ion PoSSuM
MG Magnesium ion PoSSuM
FCO Carbonmonoxide-(dicyano) iron PoSSuM
Non-standard Residues
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CSO S-hydroxycysteine
Glycosylation
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Structure summary

Code : 3CUS   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of a double ILE/PHE mutant of NI-FE hydrogenase refined at 2.2 angstrom resolution
Release Data : 2008-08-05
Compound :
mol_id molecule chains synonym
1 Periplasmic [NiFe] hydrogenase small subunit A,B,C NiFe hydrogenlyase small chain
ec: 1.12.2.1
mol_id molecule chains synonym
2 Periplasmic [NiFe] hydrogenase large subunit Q,R,S NiFe hydrogenlyase large chain
ec: 1.12.2.1
mutation: V74I, L122F
Source :
mol_id organism_scientific expression_system
1 Desulfovibrio fructosovorans  (taxid:878) Desulfovibrio fructosovorans  (taxid:878)
strain: wild type
gene: hydA
mol_id organism_scientific expression_system
2 Desulfovibrio fructosovorans  (taxid:878) Desulfovibrio fructosovorans  (taxid:878)
strain: wild type
gene: hydB
Authors : Volbeda, A.
Keywords : NI-FE hydrogenase tunnel mutant, Iron, Iron-sulfur, Metal-binding, Oxidoreductase, Nickel
Exp. method : X-RAY DIFFRACTION ( 2.20 Å )
Citation :

Experimental approaches to kinetics of gas diffusion in hydrogenase

Leroux, F.,Dementin, S.,Burlat, B.  et al.
(2008)  Proc.Natl.Acad.Sci.Usa  105 : 11188 - 11193

PubMed: 18685111
DOI: 10.1073/pnas.0803689105

Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics

Montet, Y.,Amara, P.,Volbeda, A.  et al.
(1997)  Nat.Struct.Biol.  4 : 523 - 526

PubMed: 9228943
DOI: 10.1038/nsb0797-523

High-Resolution Crystallographic Analysis of Desulfovibrio Fructosovorans [Nife] Hydrogenase

Volbeda, A.,Montet, Y.,Vernede, X.  et al.
(2002)  Int.J.Hydrogen Energy  27 : 1449 - 1461

DOI: 10.1016/S0360-3199(02)00072-1

Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases

Volbeda, A.,Martin, L.,Cavazza, C.  et al.
(2005)  J.Biol.Inorg.Chem.  10 : 239 - 249

PubMed: 15803334
DOI: 10.1007/s00775-005-0632-x

Reaction

Chain : A, B, C
UniProt : P18187 (PHNS_SOLFR)
Reaction: EC: Evidence:
Physiological Direction:
2 Fe(III)-[cytochrome c3] + H2 = 2 Fe(II)-[cytochrome c3] + 2 H(+) 1.12.2.1 -
-
Cofactor: Evidence: Note:
[4Fe-4S] cluster - Binds 2 [4Fe-4S] clusters per subunit.
[3Fe-4S] cluster - Binds 1 [3Fe-4S] cluster per subunit.
Chain : Q, R, S
UniProt : P18188 (PHNL_SOLFR)
Reaction: EC: Evidence:
Physiological Direction:
2 Fe(III)-[cytochrome c3] + H2 = 2 Fe(II)-[cytochrome c3] + 2 H(+) 1.12.2.1 -
-
Cofactor: Evidence: Note:
Ni(2+) - Binds 1 nickel ion per subunit.