Brand  (β version)

PDB ID: 3CI3

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Ligands
Code Name Style Show Link
3PO Triphosphate PoSSuM
5AD 5'-deoxyadenosine PoSSuM
B12 Cobalamin PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3CI3   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Structure of the PduO-type ATP:co(I)rrinoid adenosyltransferase from Lactobacillus reuteri complexed with partial adenosylcobalamin and PPPi
Release Data : 2008-05-27
Compound :
mol_id molecule chains
1 Cobalamin adenosyltransferase PduO-like protein A
ec: 2.5.1.17
Source :
mol_id organism_scientific expression_system
1 Lactobacillus reuteri  (taxid:1598) Escherichia coli  (taxid:562)
strain: CRL1098
gene: cobA
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28B
Authors : Maurice, M.St., Mera, P.E., Escalante-Semerena, J.C., Rayment, I.
Keywords : adenosyltransferase variant, Adenosylcobalamin binding, ATP binding, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.110 Å )
Citation :

Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate.

St Maurice, M.,Mera, P.,Park, K.  et al.
(2008)  Biochemistry  47 : 5755 - 5766

PubMed: 18452306
DOI: 10.1021/bi800132d

Reaction

Chain : A
UniProt : Q50EJ2 (Q50EJ2_LIMRT)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate 2.5.1.17 RuleBase:RU366026
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2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron- transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate 2.5.1.17 RuleBase:RU366026
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