Brand  (β version)

PDB ID: 3C4H

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Ligands
Code Name Style Show Link
DMS Dimethyl sulfoxide PoSSuM
DRL 2-methyl-3,5,7,8-tetrahydro-4h-thiopyrano[4,3-D]pyrimidin-4-one PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3C4H   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Human poly(ADP-ribose) polymerase 3, catalytic fragment in complex with an inhibitor DR2313
Release Data : 2008-02-12
Compound :
mol_id molecule chains synonym
1 Poly(ADP-ribose) polymerase 3 A PARP-3, NAD(+) ADP-ribosyltransferase 3, Poly[ADP-ribose] synthetase 3, pADPRT-3, hPARP-3, IRT1
ec: 2.4.2.30
fragment: Catalytic fragment: Residues 178-532
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli BL21(DE3)  (taxid:469008)
gene: PARP3, ADPRT3, ADPRTL3
expression_system_strain: BL21(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pNIC-Bsa4
Authors : Lehtio, L., Collins, R., Arrowsmith, C.H., Berglund, H., Bountra, C., Busam, R., Dahlgren, L.G., Edwards, A.M., Flodin, S., Flores, A., Graslund, S., Hammarstrom, M., Herman, M.D., Johansson, A., Johansson, I., Kallas, A., Karlberg, T., Kotenyova, T., Moche, M., Nilsson, M.E., Nordlund, P., Nyman, T., Persson, C., Sagemark, J., Svensson, L., Thorsell, A.G., Tresaugues, L., Van den Berg, S., Welin, M., Weigelt, J., Structural Genomics Consortium (SGC)
Keywords : enzyme-inhibitor complex, catalytic fragment, Structural Genomics, Structural Genomics Consortium, SGC, Glycosyltransferase, NAD, Nucleus, Transferase
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Structural basis for inhibitor specificity in human poly(ADP-ribose) polymerase-3.

Lehtio, L.,Jemth, A.S.,Collins, R.  et al.
(2009)  J.Med.Chem.  52 : 3108 - 3111

PubMed: 19354255
DOI: 10.1021/jm900052j

Reaction

Chain : A
UniProt : Q9Y6F1 (PARP3_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L- aspartyl-[protein] + nicotinamide - PubMed:20064938, PubMed:25043379
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L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L- glutamyl-[protein] + nicotinamide - PubMed:20064938, PubMed:25043379, PubMed:27530147
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L-lysyl-[protein] + NAD(+) = H(+) + N(6)-(ADP-D-ribosyl)-L- lysyl-[protein] + nicotinamide - PubMed:25043379
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