Brand  (β version)

PDB ID: 3BJY

Hetero Atom Contents

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Code Name Style Show Link
DCP 2'-deoxycytidine-5'-triphosphate PoSSuM
MG Magnesium ion PoSSuM

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Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
P 2'-deoxy-n1,n2-propano guanosine monophosphate
Glycosylation
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Modification
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Structure summary

Code : 3BJY   PDBj   RCSB PDB   PDBe
Header : Transferase/DNA
Title : Catalytic core of Rev1 in complex with DNA (modified template guanine) and incoming nucleotide
Release Data : 2008-10-28
Compound :
mol_id molecule chains
1 DNA repair protein REV1 A
ec: 2.7.7.-
fragment: catalytic core
mol_id molecule chains
2 DNA (5'-D(*DTP*DAP*DAP*(P)P*DGP*DTP*DAP*DGP*DGP*DGP*DGP*DAP*DGP*DGP*DAP*DT)-3') T
mol_id molecule chains
3 DNA (5'-D(*DAP*DTP*DCP*DCP*DTP*DCP*DCP*DCP*DCP*DTP*DAP*(DOC))-3') P
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Yeast Saccharomyces cerevisiae  (taxid:4932)
gene: REV1
expression_system_common: Yeast
expression_system_strain: BJ5464
expression_system_vector_type: PLASMID
expression_system_plasmid: pBJ954
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Nair, D.T., Johnson, R.E., Prakash, L., Prakash, S., Aggarwal, A.K.
Keywords : DNA Polymerase, Protein-DNA complex, adduct, bypass, DNA damage, DNA repair, DNA synthesis, DNA-binding, Magnesium, Metal-binding, Nucleotidyltransferase, Nucleus, Transferase, Transferase-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.41 Å )
Citation :

Protein-template-directed synthesis across an acrolein-derived DNA adduct by yeast Rev1 DNA polymerase

Nair, D.T.,Johnson, R.E.,Prakash, L.  et al.
(2008)  Structure  16 : 239 - 245

PubMed: 18275815
DOI: 10.1016/j.str.2007.12.009

Reaction

Chain : A
UniProt : P12689 (REV1_YEAST)
Reaction : -
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:16195463
ECO:0000269 | PubMed:18275815
ECO:0000269 | PubMed:21167175
Binds 2 magnesium ions.