Brand  (β version)

PDB ID: 3B3R

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Ligands
Code Name Style Show Link
FAE Flavin-n7 protonated-adenine dinucleotide PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 3B3R   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)
Release Data : 2007-12-18
Compound :
mol_id molecule chains synonym
1 Cholesterol oxidase A CHOD
ec: 1.1.3.6
mutation: E361Q/H447Q
other_details: FAD COFACTOR NON-COVALENTLY BOUND TO THE ENZYME
Source :
mol_id organism_scientific expression_system
1 Streptomyces sp.  (taxid:74576) Escherichia coli  (taxid:562)
strain: SA-COO
expression_system_strain: BL-21 pLys(S)
expression_system_vector_type: plasmid
expression_system_plasmid: PCO202
Authors : Lyubimov, A.Y., Heard, K., Tang, H., Sampson, N.S., Vrielink, A.
Keywords : flavoenzyme, flavin, cholesterol oxidase, covalently-modified flavin, Cholesterol metabolism, FAD, Flavoprotein, Lipid metabolism, Oxidoreductase, Secreted, Steroid metabolism
Exp. method : X-RAY DIFFRACTION ( 0.980 Å )
Citation :

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase

Lyubimov, A.Y.,Heard, K.,Tang, H.  et al.
(2007)  Protein Sci.  16 : 2647 - 2656

PubMed: 18029419
DOI: 10.1110/ps.073168207

Reaction

Chain : A
UniProt : P12676 (CHOD_STRS0)
Reaction: EC: Evidence:
Physiological Direction:
cholesterol + O2 = cholest-5-en-3-one + H2O2 1.1.3.6 PubMed:9922167
left-to-right
cholest-5-en-3-one = cholest-4-en-3-one 5.3.3.1 PubMed:9922167
left-to-right