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Ligands
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SO4 Sulfate ion
Non-standard Residues
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CR2 {(4z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid
Glycosylation
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Code : 3AI4   PDBj   RCSB PDB   PDBe
Header : Fluorescent Protein, replication
Title : Crystal structure of yeast enhanced green fluorescent protein - mouse polymerase iota ubiquitin binding motif fusion protein
Release Data : 2010-09-29
Compound :
mol_id molecule chains
1 yeast enhanced green fluorescent protein,DNA polymerase iota A
fragment: recidues 1-230 for yeast enhanced green fluorescent protein,UBIQUITIN BINDING MOTIF FUSION PROTEIN for DNA polymerase iota
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) Mouse Escherichia coli BL21(DE3)  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET28a
Authors : Suzuki, N., Wakatsuki, S., Kawasaki, M.
Keywords : UBM, Ubiquitin-binding motif, GFP, Fusion, Fluorescent Protein, replication
Exp. method : X-RAY DIFFRACTION ( 1.60 Å )
Citation :

Crystallization of small proteins assisted by green fluorescent protein

Suzuki, N.,Hiraki, M.,Yamada, Y.  et al.
(2010)  Acta Crystallogr.,Sect.D  66 : 1059 - 1066

PubMed: 20944239
DOI: 10.1107/S0907444910032944

Chain : A
UniProt : Q6R3M4 (POLI_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 PubMed:15026325
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