Het-PDB Navi2 (PDB: 3AI4)

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Ligands
Code	Name		Style	Show	Link
SO4	Sulfate ion				PoSSuM

Non-standard Residues
Code	Name	Show
CR2	{(4z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1h-imidazol-1-yl}acetic acid

Glycosylation
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Download interaction data: 3AI4

Code :

3AI4 PDBj RCSB PDB PDBe

Header :

Fluorescent Protein, replication

Title :

Crystal structure of yeast enhanced green fluorescent protein - mouse polymerase iota ubiquitin binding motif fusion protein

Release Data :

2010-09-29

Compound :

mol_id	molecule	chains
1	yeast enhanced green fluorescent protein,DNA polymerase iota	A
	fragment: recidues 1-230 for yeast enhanced green fluorescent protein,UBIQUITIN BINDING MOTIF FUSION PROTEIN for DNA polymerase iota

Source :

mol_id	organism_scientific	organism_common	expression_system
1	Mus musculus (taxid:10090)	Mouse	Escherichia coli BL21(DE3) (taxid:469008)
	expression_system_strain: BL21(DE3) expression_system_vector_type: PLASMID expression_system_plasmid: pET28a

Authors :

Suzuki, N., Wakatsuki, S., Kawasaki, M.

Keywords :

UBM, Ubiquitin-binding motif, GFP, Fusion, Fluorescent Protein, replication

Exp. method :

X-RAY DIFFRACTION ( 1.60 Å )

Citation :

Crystallization of small proteins assisted by green fluorescent protein

Suzuki, N.,Hiraki, M.,Yamada, Y. et al.
(2010) Acta Crystallogr.,Sect.D 66 : 1059 - 1066

Chain :

UniProt :

Q6R3M4 (POLI_MOUSE)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate	2.7.7.7	PubMed:15026325
	-	PubMed:15026325
Cofactor:	Evidence:	Note:
Mn(2+)	ECO:0000250 \| UniProtKB:Q9UNA4 ECO:0000250 \| UniProtKB:Q9UNA4	Binds nucleotide much more tightly and catalyzes nucleotide insertion much more efficiently in the presence of Mg(2+) than in the presence of Mn(2+).

PDB ID: 3AI4