Brand  (β version)

PDB ID: 3A6J

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Ligands
Code Name Style Show Link
CRN N-[(E)-amino(imino)methyl]-N-methylglycine PoSSuM
SO4 Sulfate ion PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3A6J   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : E122Q mutant creatininase complexed with creatine
Release Data : 2010-02-09
Compound :
mol_id molecule chains synonym
1 Creatinine amidohydrolase A,B,C,D,E,F creatininase
ec: 3.5.2.10
mutation: E122Q
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:562)
expression_system_strain: DH5alpha
expression_system_vector_type: plasmid
expression_system_plasmid: pUC19
Authors : Nakajima, Y., Yamashita, K., Ito, K., Yoshimoto, T.
Keywords : creatinine amidohydrolase, urease-related amidohydrolase superfamily, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase

Yamashita, K.,Nakajima, Y.,Matsushita, H.  et al.
(2010)  J.Mol.Biol.  396 : 1081 - 1096

PubMed: 20043918
DOI: 10.1016/j.jmb.2009.12.045

Reaction

Chain : A, B, C, D, E, F
UniProt : P83772 (CRNA_PSEPU)
Reaction: EC: Evidence:
Physiological Direction:
creatinine + H2O = creatine 3.5.2.10 PubMed:20043918, PubMed:500580
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