Brand  (β version)

PDB ID: 2XCA

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Ligands
Code Name Style Show Link
DGT 2'-deoxyguanosine-5'-triphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
8OG 8-oxo-2'-deoxy-guanosine-5'-monophosphate
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 2XCA   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : TERNARY COMPLEX OF SULFOLOBUS SOLFATARICUS DPO4 DNA POLYMERASE, 7,8- DIHYDRO-8-OXODEOXYGUANINE MODIFIED DNA AND dGTP - MAGNESIUM FORM
Release Data : 2010-09-08
Compound :
mol_id molecule chains synonym
1 DNA POLYMERASE IV A POL IV
ec: 2.7.7.7
mol_id molecule chains synonym
2 5'-D(*GP*GP*GP*GP*GP*AP*AP*GP*GP*AP*TP*TP*DOC)-3' P 13-MER PRIMER
mol_id molecule chains synonym
3 5'-D(*TP*CP*AP*CP*8OGP*GP*AP*AP*TP*CP*CP*TP*TP*CP* CP*CP*CP*C)-3' T 18-MER TEMPLATE
Source :
mol_id organism_scientific expression_system
1 SULFOLOBUS SOLFATARICUS  (taxid:273057) ESCHERICHIA COLI  (taxid:469008)
strain: P2
gene: DPO4
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET22B/DPO4-NHIS
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Irimia, A., Egli, M.
Keywords : TRANSFERASE-DNA COMPLEX, TRANSLESION NUCLEOTIDYLTRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.50 Å )
Citation :

Metal Ion Dependence of the Active Site Conformation of the Trans-Lesion DNA Polymerase Dpo4 from Sulfolobus Solfataricus

Irimia, A.,Loukachevitch, L.V.,Eoff, R.L.  et al.
(2010)  Acta Crystallogr.,Sect.F  66 : 1013

PubMed: 20823515
DOI: 10.1107/S1744309110029374

Reaction

Chain : A
UniProt : Q97W02 (DPO4_SACS2)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 -
-
Cofactor: Evidence: Note:
Mg(2+) - Binds 2 magnesium ions per subunit.