Brand  (β version)

PDB ID: 2WNF

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Ligands
Code Name Style Show Link
CG3 Hydroxy(2-hydroxyphenyl)oxoammonium PoSSuM
A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose PoSSuM
GAL Beta-D-galactopyranose PoSSuM
: Polysaccharide
Non-standard Residues
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MSE Selenomethionine
Glycosylation
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Structure summary

Code : 2WNF   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of a Mammalian Sialyltransferase in complex with Gal-beta-1-3GalNAc-ortho-nitrophenol
Release Data : 2009-10-13
Compound :
mol_id molecule chains synonym
1 CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE A GAL-NAC6S, GAL-BETA-1\,ST3GALA.1,ST3GALIA, ST3GAL I, 3-GALNAC-ALPHA-2,3-SIALYLTRANSFERASE, ST3O, SIAT4-A, BETA-GALACTOSIDE ALPHA-2\,3-SIALYLTRANSFERASE, ALPHA 2\,3-ST
ec: 2.4.99.4
fragment: RESIDUES 46-343 COMPND 12
Source :
mol_id organism_scientific organism_common expression_system
1 SUS SCROFA  (taxid:9823) PIG ESCHERICHIA COLI  (taxid:562)
expression_system_strain: ORIGAMI2
expression_system_plasmid: PCWIN2-MBP
Authors : Rao, F.V., Rich, J.R., Raikic, B., Wakarchuk, W.W., Withers, S.G., Strynadka, N.C.J.
Keywords : GLYCOSYLTRANSFERASE, DISULFIDE BOND, GOLGI APPARATUS, SIALYLTRANSFERASE, GLYCOPROTEIN, SIGNAL-ANCHOR, TRANSMEMBRANE, MEMBRANE, SECRETED, TRANSFERASE, SIALIC ACID
Exp. method : X-RAY DIFFRACTION ( 1.25 Å )
Citation :

Structural Insight Into Mammalian Sialyltransferases.

Rao, F.V.,Rich, J.R.,Rakic, B.  et al.
(2009)  Nat.Struct.Mol.Biol.  16 : 1186

PubMed: 19820709
DOI: 10.1038/NSMB.1685

Reaction

Chain : A
UniProt : Q02745 (SIA4A_PIG)
Reaction: EC: Evidence:
Physiological Direction:
a beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha- neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-alpha-D- galactosaminyl derivative + CMP + H(+) 2.4.3.4 PubMed:19820709, PubMed:8288606
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a ganglioside GM1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GD1a (d18:1(4E)) + CMP + H(+) 2.4.3.2 PubMed:8288606
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CMP-N-acetyl-beta-neuraminate + ganglioside GM1 (d18:1(4E)/18:0) = CMP + ganglioside GD1a (18:1(4E)/18:0) + H(+) - UniProtKB:P54751
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a ganglioside GA1 (d18:1(4E)) + CMP-N-acetyl-beta-neuraminate = a ganglioside GM1b (d18:1(4E)) + CMP + H(+) - PubMed:8288606
left-to-right
a ganglioside GD1b + CMP-N-acetyl-beta-neuraminate = a ganglioside GT1b + CMP + H(+) - UniProtKB:P54751
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