Brand  (β version)

PDB ID: 2WB2

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Ligands
Code Name Style Show Link
FAD Flavin-adenine dinucleotide PoSSuM
Non-standard Residues
Code Name Show
64P 5-aminothymidine 5'-(dihydrogen phosphate)
Z 1-(2-deoxy-5-O-phosphono-beta-D-erythro-pentofuranosyl)pyrimidin-2(1h)-one
Glycosylation
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Structure summary

Code : 2WB2   PDBj   RCSB PDB   PDBe
Header : LYASE/DNA
Title : Drosophila Melanogaster (6-4) Photolyase Bound To double stranded Dna containing a T(6-4)C Photolesion
Release Data : 2009-03-17
Compound :
mol_id molecule chains synonym
1 PHOTOLYASE A DNA PHOTOLYASE, RE11660P
ec: 4.1.99.3
fragment: RESIDUES 1-520
mol_id molecule chains
2 5'-D(*AP*CP*AP*GP*CP*GP*GP*64PP*ZP*GP*CP*AP *GP*GP*T)-3' C
other_details: 6-4 LINK BETWEEN T 8 AND C 8 (SEE REMARK 600)
mol_id molecule chains
3 5'-D(*TP*AP*CP*CP*TP*GP*CP*GP*AP*CP*CP*GP*CP*TP*G)-3' D
Source :
mol_id organism_scientific expression_system
1 DROSOPHILA MELANOGASTER  (taxid:7227) ESCHERICHIA COLI  (taxid:83333)
expression_system_strain: K-12
expression_system_variant: ROSETTA-GAMI PLYSS (DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: DERIVED FROM PDEST007
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific
3 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Glas, A.F., Schneider, S., Maul, M.J., Hennecke, U., Carell, T.
Keywords : LYASE-DNA COMPLEX, PHOTOLESION, DNA PHOTOLYASE, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.95 Å )
Citation :

Crystal Structure of the T(6-4)C Lesion in Complex with a (6-4) DNA Photolyase and Repair of Uv- Induced (6-4) and Dewar Photolesions.

Glas, A.F.,Schneider, S.,Maul, M.J.  et al.
(2009)  Chemistry  15 : 10387

PubMed: 19722240
DOI: 10.1002/CHEM.200901004

Crystal Structure and Mechanism of a DNA (6-4) Photolyase.

Maul, M.J.,Barends, T.R.M.,Glas, A.F.  et al.
(2008)  Angew.Chem.Int.Ed.Engl.  47 : 10076

PubMed: 18956392
DOI: 10.1002/ANIE.200804268

Reaction

Chain : A
UniProt : Q8SXK5 (Q8SXK5_DROME)
Reaction : -