Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
CA Calcium ion
DTP 2'-deoxyadenosine 5'-triphosphate
Modified Residues
Code Name Link
P 2'-deoxy-n1,n2-propano guanosine monophosphate
Code : 2R8I
Header : REPLICATION, TRANSFERASE/DNA
Title : Selectivity of Nucleoside Triphosphate Incorporation Opposite 1,N2-Propanodeoxyguanosine (PdG) by the Sulfolobus solfataricus DNA Polymerase Dpo4 Polymerase
Release Data : 2008-07-22
Compound :
mol_id molecule chains
1 DNA (5'-D(*DGP*DGP*DGP*DGP*DGP*DAP*DAP*DGP*DGP*DAP*DTP*DTP*DC)-3') P
mol_id molecule chains
2 DNA (5'-D(*DTP*DCP*DAP*DTP*(P)P*DGP*DAP*DAP*DTP*DCP*DCP*DTP*DTP*DCP*DCP*DCP*DCP*DC)-3') T
mol_id molecule chains synonym
3 DNA polymerase IV A dpo4, Pol IV
ec: 2.7.7.7
mutation: R332A
Source :
mol_id organism_scientific
1
synthetic: yes
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific expression_system
3 Sulfolobus solfataricus  (taxid:2287) Escherichia coli  (taxid:562)
gene: dbh, dpo4
other_details: purified using heat denaturation, Ni2+-nitriloacetate chromatography, and ion-exchange chromatography
Authors : Wang, Y., Saleh, S., Marnette, L.J., Egli, M., Stone, M.P.
Keywords : exocyclic adducts, replication bypass, translesion, polymerase, frameshifting, REPLICATION, TRANSFERASE-DNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.38 Å )
Citation :

Insertion of dNTPs opposite the 1,N2-propanodeoxyguanosine adduct by Sulfolobus solfataricus P2 DNA polymerase IV

Wang, Y.,Musser, S.K.,Saleh, S.  et al.
(2008)  Biochemistry  47 : 7322 - 7334

PubMed: 18563918
DOI: 10.1021/bi800152j

Chain : A
UniProt : Q97W02 (DPO4_SULSO)
Reaction : Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).