Brand  (β version)

PDB ID: 2NO5

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
SO4 Sulfate ion PoSSuM
CNR (2s)-2-chloropropanoic acid PoSSuM
Non-standard Residues
Code Name Show
ASL Aspartic acid-4-carboxyethyl ester
Glycosylation
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Structure summary

Code : 2NO5   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal Structure analysis of a Dehalogenase with intermediate complex
Release Data : 2007-09-25
Compound :
mol_id molecule chains synonym
1 (S)-2-haloacid dehalogenase IVA A,B Dehalogenase, 2-haloalkanoic acid dehalogenase IVA, L-2-haloacid dehalogenase IVA, Halocarboxylic acid halidohydrolase IVA
ec: 3.8.1.2
Source :
mol_id organism_scientific expression_system
1 Burkholderia cepacia  (taxid:292) Escherichia coli  (taxid:562)
strain: MBA4
expression_system_vector_type: plasmid
expression_system_plasmid: pHKU201
Authors : Schmidberger, J.W., Wilce, M.C.J.
Keywords : Haloacid dehalogenase, Dehalogenase, HAD Superfamily, Intermediate structure, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 2.600 Å )
Citation :

Crystal structures of the substrate free-enzyme, and reaction intermediate of the HAD superfamily member, haloacid dehalogenase DehIVa from Burkholderia cepacia MBA4

Schmidberger, J.W.,Wilce, J.A.,Tsang, J.S.H.  et al.
(2007)  J.Mol.Biol.  368 : 706 - 717

PubMed: 17368477
DOI: 10.1016/j.jmb.2007.02.015

Reaction

Chain : A, B
UniProt : Q51645 (HAD4_BURCE)
Reaction: EC: Evidence:
Physiological Direction:
an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a halide anion + H(+) 3.8.1.2 PubMed:1376111
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(S)-2-chloropropanoate + H2O = (R)-lactate + chloride + H(+) - PubMed:1376111
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