Brand  (β version)

PDB ID: 2JBV

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Ligands
Code Name Style Show Link
FAO [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2r,3s,4s)-5-[(4as,10ar)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[G]pteridin-10(2h)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate PoSSuM
DMS Dimethyl sulfoxide PoSSuM
UNX Unknown atom or ion PoSSuM
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Structure summary

Code : 2JBV   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of choline oxidase reveals insights into the catalytic mechanism
Release Data : 2007-12-25
Compound :
mol_id molecule chains
1 CHOLINE OXIDASE A,B
ec: 1.1.3.17
other_details: COVALENT LINKAGE BETWEEN C8M ATOM OF FAO AND NE2 ATOM OF HIS99 OF CHOLINE OXIDASE
Source :
mol_id organism_scientific expression_system
1 ARTHROBACTER GLOBIFORMIS  (taxid:1665) ESCHERICHIA COLI  (taxid:562)
atcc: 8010
expression_system_strain: JM109
Authors : Lountos, G.T., Fan, F., Gadda, G., Orville, A.M.
Keywords : ALCOHOL OXIDATION, FLAVOENYZME OXIDASE, COVALENTLY LINKED FAD, ARTHROBACTER GLOBIFORMIS, C4A-ADDUCT, FLAVOPROTEIN, OXIDOREDUCTASE, GLUCOSE-METHANOL-CHOLINE OXIDOREDUCTASE ENZYME SUPERFAMILY
Exp. method : X-RAY DIFFRACTION ( 1.86 Å )
Citation :

Role of Glu312 in Binding and Positioning of the Substrate for the Hydride Transfer Reaction in Choline Oxidase.

Quaye, O.,Lountos, G.T.,Fan, F.  et al.
(2008)  Biochemistry  47 : 243

PubMed: 18072756
DOI: 10.1021/BI7017943

Cloning, Sequence Analysis, and Purification of Choline Oxidase from Arthrobacter Globiformis: A Bacterial Enzyme Involved in Osmotic Stress Tolerance

Fan, F.,Ghanem, M.,Gadda, G.
(2004)  Arch.Biochem.Biophys.  421 : 149

PubMed: 14678796
DOI: 10.1016/J.ABB.2003.10.003

Kinetic Mechanism of Choline Oxidase from Arthrobacter Globiformis

Gadda, G.
(2003)  Biochim.Biophys.Acta  1646 : 112

PubMed: 12637017
DOI: 10.1016/S1570-9639(03)00003-7

The Trimethylammonium Headgroup of Choline is a Major Determinant for Substrate Binding and Specificity in Choline Oxidase

Gadda, G.,Powell, N.L.,Menon, P.
(2004)  Arch.Biochem.Biophys.  430 : 264

PubMed: 15369826
DOI: 10.1016/J.ABB.2004.07.011

On the Catalytic Mechanism of Choline Oxidase

Fan, F.,Gadda, G.
(2005)  J.Am.Chem.Soc.  127 : 2067

PubMed: 15713082
DOI: 10.1021/JA044541Q

On the Catalytic Role of the Conserved Active Site Residue His466 of Choline Oxidase

Ghanem, M.,Gadda, G.
(2005)  Biochemistry  44 : 893

PubMed: 15654745
DOI: 10.1021/BI048056J

Effects of Reversing the Protein Positive Charge in the Proximity of the Flavin N(1) Locus of Choline Oxidase

Ghanem, M.,Gadda, G.
(2006)  Biochemistry  45 : 1979

PubMed: 16519539
DOI: 10.1021/BI052514M

Reaction

Chain : A, B
UniProt : Q7X2H8 (CHOX_ARTGO)
Reaction: EC: Evidence:
Physiological Direction:
choline + H2O + 2 O2 = glycine betaine + H(+) + 2 H2O2 1.1.3.17 -
-