Brand  (β version)

PDB ID: 2IPG

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Ligands
Code Name Style Show Link
BME Beta-mercaptoethanol PoSSuM
EDO 1,2-ethanediol PoSSuM
FFA (10alpha,13alpha,14beta,17alpha)-17-hydroxyandrost-4-en-3-one PoSSuM
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
Non-standard Residues
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Structure summary

Code : 2IPG   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of 17alpha-hydroxysteroid dehydrogenase mutant K31A in complex with NADP+ and epi-testosterone
Release Data : 2007-06-19
Compound :
mol_id molecule chains
1 3(17)alpha-hydroxysteroid dehydrogenase A,B
ec: 1.1.1.-,1.1.1.209
mutation: K31A
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) Mouse Escherichia coli BL21(DE3)  (taxid:469008)
gene: Akr1c21
expression_system_strain: BL21(DE3)pLys
expression_system_vector_type: plasmid
expression_system_plasmid: pGEX1lT
Authors : Faucher, F., Cantin, L., Pereira de Jesus-Tran, K., Lemieux, M., Luu-the, V., Labrie, F., Breton, R.
Keywords : 17a-HSD, AKR1C21, AKR, aldo-keto reductase, HSD, hydroxysteroid dehydrogenase, open conformation, epi-testosterone, Oxidoreductase
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

Mouse 17alpha-Hydroxysteroid Dehydrogenase (AKR1C21) Binds Steroids Differently from other Aldo-keto Reductases: Identification and Characterization of Amino Acid Residues Critical for Substrate Binding.

Faucher, F.,Cantin, L.,Pereira de Jesus-Tran, K.  et al.
(2007)  J.Mol.Biol.  369 : 525 - 540

PubMed: 17442338
DOI: 10.1016/j.jmb.2007.03.058

Reaction

Chain : A, B
UniProt : Q91WR5 (AK1CL_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
androsterone + NADP(+) = 5alpha-androstan-3,17-dione + H(+) + NADPH 1.1.1.209 PubMed:15577209, PubMed:17442338, PubMed:20124700
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androsterone + NAD(+) = 5alpha-androstan-3,17-dione + H(+) + NADH 1.1.1.209 PubMed:15577209, PubMed:17442338, PubMed:20124700
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