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Ligands
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Non-standard Residues
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CLV {(2s)-2-[(1s)-1-aminoethyl]-5-oxo-2,5-dihydro-1h-imidazol-1-yl}acetic acid
Glycosylation
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Modification
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Code : 2HGY   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Structure of S65A Y66F E222A GFP variant after cyclization and carbon-carbon bond cleavage
Release Data : 2007-03-27
Compound :
mol_id molecule chains
1 Green fluorescent protein A
mutation: S65A, Y66F, F99S, M153T, V163A, E222A
Source :
mol_id organism_scientific expression_system
1 Aequorea victoria  (taxid:6100) Escherichia coli  (taxid:562)
gene: GFP
expression_system_strain: BL21(DE3) RIL
expression_system_vector_type: PLASMID
expression_system_plasmid: pET11a
Authors : Barondeau, D.P., Kassmann, C.J., Tainer, J.A., Getzoff, E.D.
Keywords : post-translational modification, cyclization, carbon-carbon bond cleavage, radical, GFP, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.05 Å )
Citation :

The Case of the Missing Ring: Radical Cleavage of a Carbon-Carbon Bond and Implications for GFP Chromophore Biosynthesis

Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.  et al.
(2007)  J.Am.Chem.Soc.  129 : 3118 - 3126

PubMed: 17326633
DOI: 10.1021/ja063983u

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -