Brand  (β version)

PDB ID: 2G6E

Hetero Atom Contents

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Ligands
Code Name Style Show Link
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
CRX [2-(1-aminoethyl)-2-hydroxy-4-methylene-5-oxoimidazolidin-1-yl]acetic acid
Glycosylation
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Modification
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Structure summary

Code : 2G6E   PDBj   RCSB PDB   PDBe
Header : LUMINESCENT PROTEIN
Title : Structure of cyclized F64L S65A Y66S GFP variant
Release Data : 2006-04-18
Compound :
mol_id molecule chains
1 Green fluorescent protein A
mutation: F64L, S65A, Y66S, F99S, M153T, V163A
Source :
mol_id organism_scientific expression_system
1 Aequorea victoria  (taxid:6100) Escherichia coli BL21(DE3)  (taxid:469008)
gene: GFP
expression_system_strain: BL21(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET11a
Authors : Barondeau, D.P.
Keywords : chromophore, biosynthesis, post-translational modification, histidine ammonia lyase, MIO, electrophile, LUMINESCENT PROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.30 Å )
Citation :

Understanding GFP Posttranslational Chemistry: Structures of Designed Variants that Achieve Backbone Fragmentation, Hydrolysis, and Decarboxylation.

Barondeau, D.P.,Kassmann, C.J.,Tainer, J.A.  et al.
(2006)  J.Am.Chem.Soc.  128 : 4685 - 4693

PubMed: 16594705
DOI: 10.1021/ja056635l

Reaction

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -