Brand  (β version)

PDB ID: 2DOO

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Ligands
Code Name Style Show Link
C4H N-[4-({[5-(dimethylamino)-1-naphthyl]sulfonyl}amino)butyl]-3-sulfanylpropanamide PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2DOO   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : The structure of IMP-1 complexed with the detecting reagent (DansylC4SH) by a fluorescent probe
Release Data : 2006-11-28
Compound :
mol_id molecule chains synonym
1 BETA-LACTAMASE IMP-1 A,B Beta-lactamase, type II, Penicillinase, BLAIMP
ec: 3.5.2.6
Source :
mol_id organism_scientific expression_system
1 Serratia marcescens  (taxid:615) Escherichia coli BL21(DE3)  (taxid:469008)
gene: BLAIMP-1
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET9A
expression_system_plasmid: PET9A-D-IMP
Authors : Kurosaki, H., Yamaguchi, Y., Yasuzawa, H., Jin, W., Yamagata, Y., Arakawa, Y.
Keywords : METALLO-BETA-LACTAMASE, PROTEIN-INHIBITOR COMPLEX, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.43 Å )
Citation :

Probing, inhibition, and crystallographic characterization of metallo-beta-lactamase (IMP-1) with fluorescent agents containing dansyl and thiol groups

Kurosaki, H.,Yamaguchi, Y.,Yasuzawa, H.  et al.
(2006)  Chemmedchem  1 : 969 - 972

PubMed: 16937423
DOI: 10.1002/cmdc.200600115

Reaction

Chain : A, B
UniProt : P52699 (BLBI1_SERMA)
Reaction: EC: Evidence:
Physiological Direction:
a beta-lactam + H2O = a substituted beta-amino acid 3.5.2.6 PubMed:10898670, PubMed:15788415, PubMed:15892033, PubMed:8141584
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:26482303
Binds 2 Zn(2+) ions per subunit.