Brand  (β version)

PDB ID: 2BRK

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Ligands
Code Name Style Show Link
CMF 3-cyclohexyl-1-(2-morpholin-4-yl-2-oxoethyl)-2-phenyl-1h-indole-6-carboxylic acid PoSSuM
MN Manganese (II) ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2BRK   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Hepatitis C virus polymerase in complex with an allosteric inhibitor (compound 1)
Release Data : 2005-06-14
Compound :
mol_id molecule chains synonym
1 RNA-DIRECTED RNA POLYMERASE A HEPATITIS C VIRUS POLYMERASE, P68, NS5B
ec: 2.7.7.48
fragment: NS5B CATALYTIC DOMAIN, RESIDUES 2420-2955
Source :
mol_id organism_scientific expression_system
1 HEPATITIS C VIRUS  (taxid:11103) ESCHERICHIA COLI  (taxid:469008)
strain: GENOTYPE 1B, STRAIN BK
expression_system_strain: BL21(DE3)
expression_system_vector: T7
expression_system_plasmid: PT7.7
other_details: GENBANK ACCESSION NUMBER AB016785
Authors : Di Marco, S., Volpari, C., Carfi, A.
Keywords : TRANSFERASE, HEPATITIS C VIRUS, HCV, NS5B, POLYMERASE, RNA-DEPENDENT RNA- POLYMERASE, ALLOSTERIC INHIBITOR, ATP-BINDING
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Interdomain Communication in Hepatitis C Virus Polymerase Abolished by Small-Molecule Inhibitors Bound to a Novel Allosteric Site

Di Marco, S.,Volpari, C.,Tomei, L.  et al.
(2005)  J.Biol.Chem.  280 : 29765

PubMed: 15955819
DOI: 10.1074/JBC.M505423200

Reaction

Chain : A
UniProt : P26663 (POLG_HCVBK)
Reaction: EC: Evidence:
Physiological Direction:
[Serine protease/helicase NS3]
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'. 		3.4.21.98 UniProtKB:P27958
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[Serine protease/helicase NS3]
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- diphosphate + H(+) + phosphate 		3.6.1.15 UniProtKB:P27958
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[Serine protease/helicase NS3]
ATP + H2O = ADP + H(+) + phosphate 		3.6.4.13 UniProtKB:P27958
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[RNA-directed RNA polymerase]
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 		2.7.7.48 PROSITE-ProRule:PRU00539, PubMed:20194503, PubMed:9343198
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