Brand  (β version)

PDB ID: 2ZNX

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Ligands
Code Name Style Show Link
1PG 2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol PoSSuM
Non-standard Residues
Code Name Show
FTR Fluorotryptophane
Glycosylation
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Structure summary

Code : 2ZNX   PDBj   RCSB PDB   PDBe
Header : IMMUNE SYSTEM/HYDROLASE
Title : 5-Fluorotryptophan Incorporated ScFv10 Complexed to Hen Egg Lysozyme
Release Data : 2009-01-27
Compound :
mol_id molecule chains
1 ScFv A,B
other_details: This molecule contains antibody light chain(residues 1-107), linker(residues 108-122) and antibody heavy chain(residues 123-236)
mol_id molecule chains synonym
2 Lysozyme C Y,Z 1,4-beta-N-acetylmuramidase C, Allergen Gal d 4, Allergen Gal d IV
ec: 3.2.1.17
Source :
mol_id organism_scientific organism_common expression_system
1 Synthetic construct  (taxid:32630) Mouse Escherichia coli  (taxid:562)
expression_system_strain: CT19 BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: pET30
mol_id organism_scientific organism_common
2 Gallus gallus  (taxid:9031) Chicken
tissue: egg white
Authors : DeSantis, M.E., Acchione, M., Li, M., Walter, R.L., Wlodawer, A., Smith-Gill, S.
Keywords : fluorotryptohpan, 5-fluorotryptophan, 19F, Single Chain Fv, Lysozyme, Allergen, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase, IMMUNE SYSTEM-HYDROLASE COMPLEX
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Specific fluorine labeling of the HyHEL10 antibody affects antigen binding and dynamics

Acchione, M.,Lee, Y.C.,DeSantis, M.E.  et al.
(2012)  Biochemistry  51 : 6017 - 6027

PubMed: 22769726
DOI: 10.1021/bi300455t

Reaction

Chain : Y, Z
UniProt : P00698 (LYSC_CHICK)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. 3.2.1.17 -
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