Brand  (β version)

PDB ID: 2Z2P

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Ligands
Code Name Style Show Link
DOL 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
004 (2s)-amino(phenyl)ethanoic acid
DBB D-alpha-aminobutyric acid
MHU 4-N,N-(dimethylamino)-L-phenylalanine
MHV 4-oxo-L-pipecolic acid
MHW 3-hydroxypicolinic acid
Glycosylation
Code Name Emphasize
Modification
Code Name Show
MHT (3s)-3-(methylsulfanyl)-1-azabicyclo[2.2.2]octane

Structure summary

Code : 2Z2P   PDBj   RCSB PDB   PDBe
Header : LYASE/ANTIBIOTIC
Title : Crystal Structure of catalytically inactive H270A virginiamycin B lyase from Staphylococcus aureus with Quinupristin
Release Data : 2007-06-19
Compound :
mol_id molecule chains synonym
1 Virginiamycin B lyase A,B Streptogramin B lyase
ec: 4.2.99.-
mutation: H270A
other_details: 1. SEQUENCE FOR RESIDUES 211 AND 212 WAS DERIVED FROM THE ORIGINAL GENE. 2. RESIDUES 51 TO 56 [PLPTPD] WERE REPLACED TO DISRUPT CRYSTAL PACKING INTERACTIONS BY A CORRESPONDING LOOP, RESIDUES 135-140 [ELPNKG].
mol_id molecule chains
2 Quinupristin C,D
Source :
mol_id organism_scientific expression_system
1 Staphylococcus aureus  (taxid:1280) ESCHERICHIA COLI BL21(DE3)  (taxid:469008)
strain: BM30002, PIP524
gene: vgb, vgh
expression_system_vector_type: PLASMID
expression_system_plasmid: PET15B
mol_id organism_scientific
2 STREPTOMYCES GRAMINOFACIEN  (taxid:68212)
synthetic: yes
Authors : Korczynska, M., Berghuis, A.M.
Keywords : QUINUPRISTIN, DALFOPRISTIN, STREPTOGRAMIN, SYNERCID, LYASE-ANTIBIOTIC COMPLEX, ANTIBIOTIC RESISTANCE, ANTIBIOTIC, VIRGINIAMYCIN B LYASE, VIRGINIAMYCIN B HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.80 Å )
Citation :

Structural Basis for Streptogramin B Resistance in Staphylococcus Aureus by Virginiamycin B Lyase

Korczynska, M.,Mukhtar, T.A.,Wright, G.D.  et al.
(2007)  Proc.Natl.Acad.Sci.USA  104 : 10388

PubMed: 17563376
DOI: 10.1073/PNAS.0701809104

Reaction

Chain : A, B
UniProt : P17978 (VGB_STAAU)
Reaction : -
Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:11467949
-