Brand  (β version)

PDB ID: 2Z2A

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Ligands
Code Name Style Show Link
DOR (4s)-2,6-dioxohexahydropyrimidine-4-carboxylic acid PoSSuM
NCD N-carbamoyl-L-aspartate PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
KCX Lysine nz-carboxylic acid
Glycosylation
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Structure summary

Code : 2Z2A   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Thr109Gly dihydroorotase from E. coli
Release Data : 2007-10-09
Compound :
mol_id molecule chains synonym
1 Dihydroorotase A,B DHOase
ec: 3.5.2.3
mutation: T109G
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
gene: PYRC
expression_system_strain: X7014a
expression_system_vector_type: Plasmid
expression_system_plasmid: pBluescript
Authors : Lee, M., Maher, M.J., Guss, J.M.
Keywords : TIM barrel, hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.87 Å )
Citation :

Kinetic and Structural Analysis of Mutant Escherichia coli Dihydroorotases: A Flexible Loop Stabilizes the Transition State

Lee, M.,Maher, M.J.,Christopherson, R.I.  et al.
(2007)  Biochemistry  46 : 10538 - 10550

PubMed: 17711307
DOI: 10.1021/bi701098e

Reaction

Chain : A, B
UniProt : P05020 (PYRC_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+) 3.5.2.3 HAMAP-Rule:MF_00219, PubMed:15610022, PubMed:6142052
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000255 | HAMAP-Rule:MF_00219
ECO:0000269 | PubMed:11401542
ECO:0000269 | PubMed:15610022
ECO:0000269 | PubMed:15826651
ECO:0000269 | PubMed:1671037
ECO:0000269 | PubMed:6142052
Binds 2 Zn(2+) ions per subunit (PubMed:11401542, PubMed:15826651, PubMed:6142052). In vitro, can also use Co(2+) or Cd(2+) (PubMed:15610022, PubMed:1671037).