Brand  (β version)

PDB ID: 2YK7

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
1PE Pentaethylene glycol PoSSuM
CSF Cytidine-5'-monophosphate-3-fluoro-N-acetyl-neuraminic acid PoSSuM
EDO 1,2-ethanediol PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 2YK7   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Structure of Neisseria LOS-specific sialyltransferase (NST), in complex with CMP-3F-Neu5Ac.
Release Data : 2011-08-31
Compound :
mol_id molecule chains synonym
1 CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE A ALPHA 2\,3-ST, BETA-GALACTOSIDE ALPHA-2\,3-SIALYL TRANSFERASE, LIPOOLIGOSACCHARIDE SIALYLTRANSFERASE, LOS-SPECIFIC SIALYLTRANSFERASE
ec: 2.4.99.-
fragment: DELTA29NST, RESIDUES 49-370
Source :
mol_id organism_scientific expression_system
1 NEISSERIA MENINGITIDIS SEROGROUP B  (taxid:491) ESCHERICHIA COLI  (taxid:469008)
strain: 126E / NRCC4010
variant: L1 IMMUNOTYPE
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET-41B
Authors : Lin, L.Y.C., Rakic, B., Chiu, C.P.C., Lameignere, E., Wakarchuk, W.W., Withers, S.G., Strynadka, N.C.J.
Keywords : TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.18 Å )
Citation :

Structure and Mechanism of the Lipooligosaccharide Sialyltransferase from Neisseria Meningitidis

Lin, L.Y.C.,Rakic, B.,Chiu, C.P.C.  et al.
(2011)  J.Biol.Chem.  286 : 37237

PubMed: 21880735
DOI: 10.1074/JBC.M111.249920

Reaction

Chain : A
UniProt : P72097 (LST_NEIMB)
Reaction: EC: Evidence:
Physiological Direction:
a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha- neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D- glucosaminyl derivative + CMP + H(+) 2.4.3.6 PubMed:8910446
-