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Code : 2Y8Y   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/RNA
Title : Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA
Release Data : 2011-05-18
Compound :
mol_id molecule chains synonym
1 CSE3 A TTHB192
mol_id molecule chains
2 5'-R(*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP *GP*GP*DGP*AP*U)-3' B
other_details: RNA WITH 2'DEOXY MODIFICATION AT G 21
Source :
mol_id organism_scientific expression_system
1 THERMUS THERMOPHILUS  (taxid:300852) ESCHERICHIA COLI  (taxid:469008)
strain: HB8
atcc: 27634
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PSV272 - HIS6-MBP-TEV
mol_id organism_scientific
2
synthetic: yes
Authors : Sashital, D.G., Jinek, M., Doudna, J.A.
Keywords : HYDROLASE-RNA COMPLEX, FERREDOXIN-LIKE
Exp. method : X-RAY DIFFRACTION ( 1.44 Å )
Citation :

An RNA-Induced Conformational Change Required for Crispr RNA Cleavage by the Endoribonuclease Cse3.

Sashital, D.G.,Jinek, M.,Doudna, J.A.
(2011)  Nat.Struct.Mol.Biol.  18 : 680

PubMed: 21572442
DOI: 10.1038/NSMB.2043

Chain : A
UniProt : Q53WG9 (CAS6_THET8)
Reaction : -