Brand  (β version)

PDB ID: 2XJB

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Ligands
Code Name Style Show Link
DG 2'-deoxyguanosine-5'-monophosphate PoSSuM
DTP 2'-deoxyadenosine 5'-triphosphate PoSSuM
GOL Glycerol PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 2XJB   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II in complex with deoxyguanosine monophosphate and deoxyadenosine triphosphate
Release Data : 2011-03-16
Compound :
mol_id molecule chains synonym
1 CYTOSOLIC PURINE 5'-NUCLEOTIDASE A CYTOSOLIC 5'-NUCLEOTIDASE II
ec: 3.1.3.5
fragment: RESIDUES 1-536
mutation: YES
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_variant: R3-PRARE2
expression_system_vector: P28A-LIC
Authors : Wallden, K., Nordlund, P.
Keywords : ALLOSTERIC ENZYME, CN-II, GMP-IMP SPECIFIC NUCLEOTIDASE, HIGH KM 5-PRIME NUCLEOTIDASE, HYDROLASE, METAL-BINDING, NT5C2, NUCLEOTIDE METABOLISM, NUCLEOTIDE-BINDING, PHOSPHOPROTEIN
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Structural Basis for the Allosteric Regulation and Substrate Recognition of Human Cytosolic 5'-Nucleotidase II

Wallden, K.,Nordlund, P.
(2011)  J.Mol.Biol.  408 : 684

PubMed: 21396942
DOI: 10.1016/J.JMB.2011.02.059

Reaction

Chain : A
UniProt : P49902 (5NTC_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate 3.1.3.5 PubMed:10092873, PubMed:12907246, PubMed:1659319, PubMed:17405878, PubMed:9371705
left-to-right PubMed:1659319
a 2'-deoxyribonucleoside + a ribonucleoside 5'-phosphate = a 2'-deoxyribonucleoside 5'-phosphate + a ribonucleoside 2.7.1.77 PubMed:1659319, PubMed:9371705
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H2O + IMP = inosine + phosphate 3.1.3.99 PubMed:10092873, PubMed:12907246, PubMed:1659319, PubMed:9371705
left-to-right PubMed:1659319
GMP + H2O = guanosine + phosphate - UniProtKB:D3ZMY7
left-to-right
dIMP + H2O = 2'-deoxyinosine + phosphate - UniProtKB:D3ZMY7
left-to-right
dGMP + H2O = 2'-deoxyguanosine + phosphate - PubMed:12907246, PubMed:1659319
left-to-right PubMed:1659319
H2O + XMP = phosphate + xanthosine - UniProtKB:D3ZMY7
left-to-right
GMP + inosine = guanosine + IMP - PubMed:1659319, PubMed:9371705
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dGMP + inosine = 2'-deoxyguanosine + IMP - PubMed:1659319, PubMed:9371705
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dIMP + inosine = 2'-deoxyinosine + IMP - PubMed:1659319, PubMed:9371705
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inosine + UMP = IMP + uridine - PubMed:1659319, PubMed:9371705
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CMP + inosine = cytidine + IMP - PubMed:1659319, PubMed:9371705
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AMP + inosine = adenosine + IMP - PubMed:1659319, PubMed:9371705
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