Brand  (β version)

PDB ID: 2XCT

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Ligands
Code Name Style Show Link
MN Manganese (II) ion PoSSuM
CPF 1-cyclopropyl-6-fluoro-4-oxo-7-piperazin-1-yl-1,4-dihydroquinoline-3-carboxylic acid PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2XCT   PDBj   RCSB PDB   PDBe
Header : ISOMERASE/DNA/ANTIBIOTIC
Title : The twinned 3.35A structure of S. aureus Gyrase complex with Ciprofloxacin and DNA
Release Data : 2010-08-25
Compound :
mol_id molecule chains
1 DNA GYRASE SUBUNIT B, DNA GYRASE SUBUNIT A B,D,S,U
ec: 5.99.1.3
fragment: GYRB- C-TERMINAL 27KDA DOMAIN, RESIDUES 410-543 AND 580-644, GYRA- N-TERMINAL 56KDA DOMAIN, RESIDUES 2-491
mutation: YES
other_details: C-TERMINUS GYRB (644) FUSED TO N-TERMINUS GYRA (1002). GREEK KEY DOMAIN (544-579) DELETED AND REPLACED WITH TWO RESIDUES, TG
mol_id molecule chains
2 5'-D(TP*GP*TP*GP*CP*GP*GP*T)-3' E,V
mol_id molecule chains
3 5'-D(AP*GP*CP*CP*GP*TP*AP*G)-3' F,W
mol_id molecule chains
4 5'-D(GP*TP*AP*CP*CP*CP*AP*CP*GP*GP*CP*T)-3' G,X
mol_id molecule chains
5 5'-D(GP*TP*AP*CP*AP*CP*CP*GP*CP*AP*CP*A)-3' H,Y
Source :
mol_id organism_scientific expression_system
1 STAPHYLOCOCCUS AUREUS  (taxid:158879) ESCHERICHIA COLI  (taxid:469008)
strain: N315
expression_system_strain: BL21(DE3)
mol_id organism_scientific
2 SYNTHETIC CONSTRUCT  (taxid:32630)
synthetic: yes
mol_id organism_scientific
3 SYNTHETIC CONSTRUCT  (taxid:32630)
synthetic: yes
mol_id organism_scientific
4 SYNTHETIC CONSTRUCT  (taxid:32630)
synthetic: yes
mol_id organism_scientific
5 SYNTHETIC CONSTRUCT  (taxid:32630)
synthetic: yes
Authors : Bax, B.D., Chan, P., Eggleston, D.S., Fosberry, A., Gentry, D.R., Gorrec, F., Giordano, I., Hann, M.M., Hennessy, A., Hibbs, M., Huang, J., Jones, E., Jones, J., Brown, K.K., Lewis, C.J., May, E., Singh, O., Spitzfaden, C., Shen, C., Shillings, A., Theobald, A., Wohlkonig, A., Pearson, N.D., Gwynn, M.N.
Keywords : ISOMERASE-DNA-ANTIBIOTIC COMPLEX
Exp. method : X-RAY DIFFRACTION ( 3.35 Å )
Citation :

Type Iia Topoisomerase Inhibition by a New Class of Antibacterial Agents.

Bax, B.D.,Chan, P.F.,Eggleston, D.S.  et al.
(2010)  Nature  466 : 935

PubMed: 20686482
DOI: 10.1038/NATURE09197

Reaction

Chain : B, D, S, U
UniProt : P66937 (GYRB_STAAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP-dependent breakage, passage and rejoining of double- stranded DNA. 5.6.2.2 HAMAP- Rule:MF_01898
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Chain : B, D, S, U
UniProt : Q99XG5 (GYRA_STAAN)
Reaction: EC: Evidence:
Physiological Direction:
ATP-dependent breakage, passage and rejoining of double- stranded DNA. 5.6.2.2 HAMAP- Rule:MF_01897
-