Brand  (β version)

PDB ID: 2WU5

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Ligands
Code Name Style Show Link
FAD Flavin-adenine dinucleotide PoSSuM
TEO Malate like intermediate PoSSuM
NA Sodium ion PoSSuM
FES Fe2/s2 (inorganic) cluster PoSSuM
SF4 Iron/sulfur cluster PoSSuM
F3S Fe3-s4 cluster PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
CBE 2-methyl-N-phenyl-5,6-dihydro-1,4-oxathiine-3-carboxamide PoSSuM
Non-standard Residues
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Structure summary

Code : 2WU5   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhD His71Met mutant
Release Data : 2010-08-25
Compound :
mol_id molecule chains
1 SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT A,E,I
ec: 1.3.5.1, 1.3.99.1
other_details: FAD ATOM C8M IS COVALENTLY LINKED TO NE2 OF SDHA HIS45
mol_id molecule chains
2 SUCCINATE DEHYDROGENASE IRON-SULFUR SUBUNIT B,F,J
ec: 1.3.5.1, 1.3.99.1
mol_id molecule chains synonym
3 SUCCINATE DEHYDROGENASE CYTOCHROME B556 SUBUNIT C,G,K CYTOCHROME B-556
ec: 1.3.5.1
other_details: RESIDUES 8-129 MODELLED
mol_id molecule chains
4 SUCCINATE DEHYDROGENASE HYDROPHOBIC MEMBRANE ANCHOR PROTEIN SUBUNIT D,H,L
ec: 1.3.5.1
mutation: YES
other_details: RESIDUES 11-115 MODELLED
Source :
mol_id organism_scientific expression_system
1 ESCHERICHIA COLI  (taxid:562) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: DW35
expression_system_vector_type: PLASMID
expression_system_plasmid: PFAS
mol_id organism_scientific expression_system
2 ESCHERICHIA COLI  (taxid:562) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: DW35
expression_system_vector_type: PLASMID
expression_system_plasmid: PFAS
mol_id organism_scientific expression_system
3 ESCHERICHIA COLI  (taxid:562) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: DW35
expression_system_vector_type: PLASMID
expression_system_plasmid: PFAS
mol_id organism_scientific expression_system
4 ESCHERICHIA COLI  (taxid:562) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: DW35
expression_system_vector_type: PLASMID
expression_system_plasmid: PFAS
Authors : Ruprecht, J., Yankovskaya, V., Maklashina, E., Iwata, S., Cecchini, G.
Keywords : CELL INNER MEMBRANE, TRICARBOXYLIC ACID CYCLE, METAL-BINDING, TRANSMEMBRANE, TRANSPORT, FLAVOPROTEIN, OXIDOREDUCTASE, ELECTRON TRANSPORT
Exp. method : X-RAY DIFFRACTION ( 2.803 Å )
Citation :

Crystal Structure of the E. Coli Succinate:Quinone Oxidoreductase (Sqr) Sdhd His71met Mutant

Ruprecht, J.,Yankovskaya, V.,Maklashina, E.  et al.
To be Published 

Reaction

Chain : A, E, I
UniProt : P0AC41 (SDHA_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
a quinone + succinate = a quinol + fumarate 1.3.5.1 PubMed:19710024
-
Chain : B, F, J
UniProt : P07014 (SDHB_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
a quinone + succinate = a quinol + fumarate 1.3.5.1 -
-
Chain : C, G, K
UniProt : P69054 (DHSC_ECOLI)
Reaction : -
Chain : D, H, L
UniProt : P0AC44 (DHSD_ECOLI)
Reaction : -