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Ligands
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Non-standard Residues
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CRF [(4z)-2-[(1r,2r)-1-amino-2-hydroxypropyl]-4-(1h-indol-3-ylmethylidene)-5-oxo-4,5-dihydro-1h-imidazol-1-yl]acetic acid
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Code : 2WSN   PDBj   RCSB PDB   PDBe
Header : FLUORESCENT PROTEIN
Title : Structure of Enhanced Cyan Fluorescent Protein at physiological pH
Release Data : 2009-09-29
Compound :
mol_id molecule chains
1 GREEN FLUORESCENT PROTEIN A
fragment: RESIDUES 2-238
other_details: CHROMOPHORE RESULTING FROM AUTOCATALYTIC CYCLIZATION OF CONSECUTIVE AMINO ACID RESIDUES THR65, TRP66 AND GLY67.
Source :
mol_id organism_scientific organism_common expression_system
1 AEQUOREA VICTORIA  (taxid:6100) JELLYFISH ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21 CODONPLUS(DE3) RIL
expression_system_vector: PQE60
Authors : Lelimousin, M., Noirclerc-Savoye, M., Lazareno-Saez, C., Paetzold, B., Le Vot, S., Chazal, R., Macheboeuf, P., Field, M.J., Bourgeois, D., Royant, A.
Keywords : FLUORESCENT PROTEIN, CHROMOPHORE, LUMINESCENCE, PHOTOPROTEIN
Exp. method : X-RAY DIFFRACTION ( 1.37 Å )
Citation :

Intrinsic Dynamics in Ecfp and Cerulean Control Fluorescence Quantum Yield.

Lelimousin, M.,Noirclerc-Savoye, M.,Lazareno-Saez, C.  et al.
(2009)  Biochemistry  48 : 10038

PubMed: 19754158
DOI: 10.1021/BI901093W

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins.

Bae, J.H.,Rubini, M.,Jung, G.  et al.
(2003)  J.Mol.Biol.  328 : 1071

PubMed: 12729742

Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -