Brand  (β version)

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interaction residue:

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Ligands
Code Name Link
CA Calcium ion
ZN Zinc ion
023 N^2^-[(2r)-2-{(1s)-1-[formyl(hydroxy)amino]ethyl}-5-phenylpentanoyl]-N,3-dimethyl-L-valinamide
077 (3s)-5-biphenyl-4-yl-3-hydroxypentanoic acid
GOL Glycerol
SO4 Sulfate ion
Code : 2WO8
Header : HYDROLASE
Title : MMP12 complex with a beta hydroxy carboxylic acid
Release Data : 2009-09-08
Compound :
mol_id molecule chains synonym
1 MACROPHAGE METALLOELASTASE A,B,C,D MATRIX METALLOPROTEINASE-12, MMP12, MACROPHAGE METALLOELASTASE, MACROPHAGE ELASTASE, ME
ec: 3.4.24.65
fragment: CATALYTIC DOMAIN, RESIDUES 106-268
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:562)
cell: MACROPHAGE
expression_system_strain: BLR(DE3)
expression_system_plasmid: PET11A
Authors : Holmes, I.P., Gaines, S., Watson, S.P., Lorthioir, O., Walker, A., Baddeley, S.J., Herbert, S., Egan, D., Convery, M.A., Singh, O.M.P., Gross, J.W., Strelow, J.M., Smith, R.H., Amour, A.J., Brown, D., Martin, S.L.
Keywords : HYDROLASE, POLYMORPHISM, GLYCOPROTEIN, METAL-BINDING, MATRIX METALLOPROTEASE MMP-12 COMPLEX STRUCTURE, METALLOPROTEASE, EXTRACELLULAR MATRIX, ZYMOGEN, PROTEASE, SECRETED
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

The Identification of Beta-Hydroxy Carboxylic Acids as Selective Mmp-12 Inhibitors.

Holmes, I.P.,Gaines, S.,Watson, S.P.  et al.
(2009)  Bioorg.Med.Chem.Lett.  19 : 5760

PubMed: 19703773
DOI: 10.1016/J.BMCL.2009.07.155

Chain : A, B, C, D
UniProt : P39900 (MMP12_HUMAN)
Reaction : Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16- Tyr-|-Leu-17 in the B chain of insulin.