Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
CG3 Hydroxy(2-hydroxyphenyl)oxoammonium
Modified Residues
Code Name Link
A2G 2-acetamido-2-deoxy-alpha-D-galactopyranose
GAL Beta-D-galactopyranose
MSE Selenomethionine
Code : 2WNF
Header : TRANSFERASE
Title : Crystal Structure of a Mammalian Sialyltransferase in complex with Gal-beta-1-3GalNAc-ortho-nitrophenol
Release Data : 2009-10-13
Compound :
mol_id molecule chains synonym
1 CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE A GAL-NAC6S, GAL-BETA-1\,ST3GALA.1,ST3GALIA, ST3GAL I, 3-GALNAC-ALPHA-2,3-SIALYLTRANSFERASE, ST3O, SIAT4-A, BETA-GALACTOSIDE ALPHA-2\,3-SIALYLTRANSFERASE, ALPHA 2\,3-ST
ec: 2.4.99.4
fragment: RESIDUES 46-343 COMPND 12
Source :
mol_id organism_scientific organism_common expression_system
1 SUS SCROFA  (taxid:9823) PIG ESCHERICHIA COLI  (taxid:562)
expression_system_strain: ORIGAMI2
expression_system_plasmid: PCWIN2-MBP
Authors : Rao, F.V., Rich, J.R., Raikic, B., Wakarchuk, W.W., Withers, S.G., Strynadka, N.C.J.
Keywords : GLYCOSYLTRANSFERASE, DISULFIDE BOND, GOLGI APPARATUS, SIALYLTRANSFERASE, GLYCOPROTEIN, SIGNAL-ANCHOR, TRANSMEMBRANE, MEMBRANE, SECRETED, TRANSFERASE, SIALIC ACID
Exp. method : X-RAY DIFFRACTION ( 1.25 Å )
Citation :

Structural Insight Into Mammalian Sialyltransferases.

Rao, F.V.,Rich, J.R.,Rakic, B.  et al.
(2009)  Nat.Struct.Mol.Biol.  16 : 1186

PubMed: 19820709
DOI: 10.1038/NSMB.1685

Chain : A
UniProt : Q02745 (SIA4A_PIG)
Reaction : CMP-N-acetylneuraminate + beta-D-galactosyl- 1,3-N-acetyl-alpha-D-galactosaminyl-R = CMP + alpha-N- acetylneuraminyl-2,3-beta-D-galactosyl-1,3-N-acetyl-alpha-D- galactosaminyl-R.