Brand  (β version)

PDB ID: 2WB4

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Ligands
Code Name Style Show Link
BEF Beryllium trifluoride ion PoSSuM
C2E 9,9'-[(2r,3r,3as,5s,7ar,9r,10r,10as,12s,14ar)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2h,7h-difuro[3,2-D:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6h-purin-6-one) PoSSuM
MG Magnesium ion PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2WB4   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : activated diguanylate cyclase PleD in complex with C-di-GMP
Release Data : 2010-04-21
Compound :
mol_id molecule chains synonym
1 DIGUANYLATE CYCLASE A,B STALKED CELL DIFFERENTIATION-CONTROLLING PROTEIN, RESPONSE REGULATOR PLED, DGC, PLED
ec: 2.7.7.65, 4.6.1.-
other_details: BERRYLIUM FLUORIDE MODIFICATION OF A 53 AND B 53
Source :
mol_id organism_scientific expression_system
1 CAULOBACTER CRESCENTUS  (taxid:190650) ESCHERICHIA COLI  (taxid:469008)
strain: CB15
expression_system_strain: BL21(DE3)PLYSS
expression_system_plasmid: PRUN
Authors : Wassmann, P., Schirmer, T.
Keywords : CELL CYCLE, TRANSFERASE, GTP-BINDING, METAL-BINDING, PHOSPHOPROTEIN, DIFFERENTIATION, RESPONSE REGULATOR, BEF3-, C-DI-GMP, MAGNESIUM, TRANSDUCER, TWO-COMPONENT REGULATORY SYSTEM, NUCLEOTIDE-BINDING, DIGUANYLATE CYCLASE
Exp. method : X-RAY DIFFRACTION ( 2.80 Å )
Citation :

Crystal Structure of Activated Pled, Identification of Dimerization and Catalysis Relevant Regulatory Mechanisms

Wassmann, P.,Massa, C.,Zaehringer, F.  et al.
To be Published 

Reaction

Chain : A, B
UniProt : Q9A5I5 (PLED_CAUVC)
Reaction: EC: Evidence:
Physiological Direction:
2 GTP = 3',3'-c-di-GMP + 2 diphosphate 2.7.7.65 -
-