Brand  (β version)

PDB ID: 2WA4

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Ligands
Code Name Style Show Link
069 N,3-dihydroxybenzamide PoSSuM
FE2 Fe (II) ion PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 2WA4   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : FACTOR INHIBITING HIF-1 ALPHA WITH N,3-dihydroxybenzamide
Release Data : 2010-04-21
Compound :
mol_id molecule chains synonym
1 HYPOXIA-INDUCIBLE FACTOR 1-ALPHA INHIBITOR A FACTOR INHIBITING HIF1, HYPOXIA-INDUCIBLE FACTOR ASPARAGINE HYDROXYLASE, FACTOR INHIBITING HIF-1, FIH-1
ec: 1.14.11.16
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:469008)
expression_system_strain: BL21(DE3)
expression_system_plasmid: PET28A
Authors : Conejo-Garcia, A., Lienard, B.M.R., Clifton, I.J., McDonough, M.A., Schofield, C.J.
Keywords : HYDROXYLASE, DIOXYGENASE, TRANSCRIPTION, OXIDOREDUCTASE, TRANSCRIPTION ACTIVATOR/INHIBITOR, HYPOXIA
Exp. method : X-RAY DIFFRACTION ( 2.50 Å )
Citation :

Structural basis for binding of cyclic 2-oxoglutarate analogues to factor-inhibiting hypoxia-inducible factor.

Conejo-Garcia, A.,McDonough, M.A.,Loenarz, C.  et al.
(2010)  Bioorg. Med. Chem. Lett.  20 : 6125 - 6128

PubMed: 20822901
DOI: 10.1016/j.bmcl.2010.08.032

Structure of Factor-Inhibiting Hypoxia-Inducible Factor (Hif) Reveals Mechanism of Oxidative Modification of Hif-1Alpha

Elkins, J.,Hewitson, K.S.,McNeill, L.  et al.
(2003)  J.Biol.Chem.  278 : 1802

PubMed: 12446723
DOI: 10.1074/JBC.C200644200

Reaction

Chain : A
UniProt : Q9NWT6 (HIF1N_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2-oxoglutarate + L-asparaginyl-[hypoxia-inducible factor alpha subunit] + O2 = (3S)-3-hydroxy-L-asparaginyl-[hypoxia-inducible factor alpha subunit] + CO2 + succinate 1.14.11.30 PubMed:12042299, PubMed:12080085, PubMed:14701857, PubMed:17573339
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2-oxoglutarate + L-histidyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-histidyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:21251231
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2-oxoglutarate + L-asparaginyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-asparaginyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:17003112, PubMed:17573339, PubMed:18299578, PubMed:19245366, PubMed:21177872
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2-oxoglutarate + L-aspartyl-[ankyrin-repeat domain protein] + O2 = (3S)-3-hydroxy-L-aspartyl-[ankyrin-repeat domain protein] + CO2 + succinate 1.14.11.n4 PubMed:21177872
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